Experiment Overview
| Repository ID: | FR-FCM-Z3ZD | Experiment name: | Methamphetamine Use Alters Human Plasma Extracellular Vesicles | MIFlowCyt score: | 60.25% |
| Primary researcher: | John Nolan | PI/manager: | John Nolan | Uploaded by: | John Nolan |
| Experiment dates: | 2018-01-01 - 2020-08-31 | Dataset uploaded: | Sep 2020 | Last updated: | Sep 2020 |
| Keywords: | [miRNA] [exosome] [microvesicle] [extracellular vesicle] [methamphetamine] [plasma] | Manuscripts: | [33613872] [PMC7890470] | ||
| Organizations: |
Scintillon Institute, San Diego, CA (USA)
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| Purpose: | The purpose of this study was to measure extracellular vesicles (EVs) and their cargo in plasma from methamphetamine users and control subjects. | ||||
| Conclusion: | None | ||||
| Comments: | None | ||||
| Funding: | This work was supported by the MARC under grant P50 DA018165 and Department of Veterans Affairs, Veterans Health Administration, Office of Research and Development, Biomedical Laboratory Research and Development Merit Review Awards [1I01BX002061 (JML), I01BX004934 (AJ)] and Clinical Sciences Research and Development Merit Review Award [1I01CX001592-01 (MH)]. | ||||
| Quality control: | Instrument performance was characterized using a combination of multi-intensity single fluorophore beads (Quantum FITC, Bangs Labs Quantibrite PE, BD Biosciences) whose intensity had been calibrated in units of MESF, multi-intensity multifluorophore beads (vCal nanoRainbow, Cellarcus), and antibody capture beads (vCal antibody capture beads, Cellarcus) calibrated to report results in units of antibodies bound per vesicle (ABV). EV analysis by vesicle flow cytometry (VFC) was conducted and reported as suggested by the MIFlowCyt-EV guidelines (See attached checklist. | ||||
