Experiment Overview
| Repository ID: | FR-FCM-Z43R | Experiment name: | SEGA library analysis and sorting | MIFlowCyt score: | 31.50% |
| Primary researcher: | Maja Rennig | PI/manager: | Maja Rennig | Uploaded by: | Maja Rennig |
| Experiment dates: | 2017-06-01 - 2021-09-01 | Dataset uploaded: | Jun 2021 | Last updated: | Sep 2021 |
| Keywords: | [E. coli] [Synthetic Biology] [genome engineering] [strain collection] [Expression libraries] | Manuscripts: | |||
| Organizations: |
Technical University of Denmark, Technical University of Denmark, Kgs Lyngby, (Denmark)
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| Purpose: | This experiment was performed to isolate low, medium and high expressing variants of E. coli MG1655 J23100-middle gadget-GFP to construct the SEGA strain collection. Low expressing variants were selected below the 5th percentile, high expressing variants were isolated above the 95th percentile. Medium expressing variants were selected from a gate that was set in between the low and high expression gate. | ||||
| Conclusion: | Libraries with sufficient quality were constructed on the genome of E. coli MG1655 and could be sorted into low, medium and high expressing TIR variants. | ||||
| Comments: | None | ||||
| Funding: | The authors acknowledge funding by the Novo Nordisk Foundation (NNF20CC0035580) and by the “Bioroboost” project under EU Horizon 2020 research and innovation programme under grant agreement N820699. AKE was supported by grant no. NNF18CC0033664 as a fellow of the Copenhagen Bioscience PhD Programme. | ||||
| Quality control: | Wildtype MG1655 cells were used as negative control. MG1655 J23100-BCD-GFP was used as positive control. | ||||
