Experiment Overview
| Repository ID: | FR-FCM-Z45M | Experiment name: | GFP-RNF152 degradation depends on lysosomes | MIFlowCyt score: | 18.00% |
| Primary researcher: | Weichao Zhang | PI/manager: | Ming Li | Uploaded by: | Weichao Zhang |
| Experiment dates: | 2019-04-30 - | Dataset uploaded: | Jun 2021 | Last updated: | Jun 2021 |
| Keywords: | None | Manuscripts: | |||
| Organizations: | None | ||||
| Purpose: | To determine which pathway, lysosome or proteasome, is responsible for the degradation of GFP-RNF152. | ||||
| Conclusion: | The degradation of GFP-RNF152 depends on lysosomes. | ||||
| Comments: | MG132 is a proteasome inhibitor. BafA1 is vATPase inhibitor, which block lysosome function. Cells were treated with DMSO, MG132, BafA1 or MG132+BafA1 for 6 hours. GFP-RNF152 is stably expressed in the cell. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
