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Experiment: CHMP4A+4B knockdown increases GFP-RNF152 level.
ID: FR-FCM-Z45N
Primary Researcher: Weichao Zhang
MIFlowCyt Score: 18.00%

Experiment Overview

Repository ID:	FR-FCM-Z45N	Experiment name:	CHMP4A+4B knockdown increases GFP-RNF152 level.	MIFlowCyt score:	18.00%
Primary researcher:	Weichao Zhang	PI/manager:	Ming Li	Uploaded by:	Weichao Zhang
Experiment dates:	2020-08-27 -	Dataset uploaded:	Jun 2021	Last updated:	Jun 2021
Keywords:	None		Manuscripts:
Organizations:	None
Purpose:	To determine whether the degradation of GFP-RNF152 depends on the ESCRT machinery.
Conclusion:	CHMP4A+4B knockdown increases GFP-RNF152 level.
Comments:	Cells were transfected with control siRNA or siRNAs against CHMP4A and CHMP4B for 72 hours. GFP-RNF152 is stably expressed in the cell.
Funding:	Not disclosed
Quality control:	None

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The following open access article describes how to upload and annotate flow cytometry data sets: Spidlen J, Breuer K and Brinkman R. Preparing a Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) Compliant Manuscript Using the International Society for Advancement of Cytometry (ISAC) FCS File Repository (FlowRepository.org). Current Protocols in Cytometry, UNIT 10.18, July 2012.
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You may download slides from our Workshop at CYTO 2012: Publishing MIFlowCyt Compliant Data to ISAC’s FlowRepository.org for Cytometry A and Other Journals
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Citing FlowRepository

Please reference us by citing:

Spidlen J, Breuer K, Rosenberg C, Kotecha N and Brinkman RR. FlowRepository - A Resource of Annotated Flow Cytometry Datasets Associated with Peer-reviewed Publications. Cytometry A. 2012 Sep; 81(9):727-31..

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