Experiment Overview
| Repository ID: | FR-FCM-Z45W | Experiment name: | Plant tissue selection for nuclear DNA content estimation | MIFlowCyt score: | 81.75% |
| Primary researcher: | Martin Čertner | PI/manager: | Martin Čertner | Uploaded by: | Martin Čertner |
| Experiment dates: | 2020-10-13 - 2020-12-04 | Dataset uploaded: | Jun 2021 | Last updated: | Jun 2021 |
| Keywords: | [plant tissue selection] [Solanum pseudocapsicum] [DNA content estimation] [endopolyploidy] [secondary metabolites] | Manuscripts: | |||
| Organizations: |
Institute of Botany of the Czech Academy of Sciences, Průhonice, (Czech Republic)
|
||||
| Purpose: | A figure illustrating the differences in FCM histograms when alternative organ/tissue types of the same plant were used for DNA content estimation. | ||||
| Conclusion: | Organ / tissue type selection can be used to lower the risk of incorrect DNA content estimates caused by high content of secondary metabolites interfering with fluorescent staining and/or tissue endopolyploidy. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | To maximize comparability, all tissue samples were collected from the same individual and analyzed on the same instrument. Lower quality of some FCM histograms was expected in the experiment (and reproduced in an unaltered form). | ||||
Experiment variables
| Sample Type | |
|---|---|
| · Fruit skin | Solanum_pseudocapsicum_fruit-skin.FCS |
| · Mature leaf | Solanum_pseudocapsicum_mature-leaf.FCS |
| · Petal blades | Solanum_pseudocapsicum_petal-blades.FCS |
| · Root | Solanum_pseudocapsicum_root.FCS |
| · Stem | Solanum_pseudocapsicum_stem.FCS |
| · Young leaf | Solanum_pseudocapsicum_young-leaf.FCS |
