Experiment Overview
| Repository ID: | FR-FCM-Z45X | Experiment name: | Overexpression of VPS4A E228Q increases GFP-RNF152 level. | MIFlowCyt score: | 38.00% |
| Primary researcher: | Weichao Zhang | PI/manager: | Ming Li | Uploaded by: | Weichao Zhang |
| Experiment dates: | 2020-09-18 - | Dataset uploaded: | Jun 2021 | Last updated: | Jun 2021 |
| Keywords: | None | Manuscripts: | |||
| Organizations: | None | ||||
| Purpose: | To determine whether the degradation of GFP-RNF152 depends on VPS4 AAA-ATPase. | ||||
| Conclusion: | Overexpression of the dominant-negative mutant of VPS4A (E228Q) leads to the accumulation of GFP-RNF152. | ||||
| Comments: | The expression of VPS4A WT and E228 is driven by a Tet-on promoter. The expression is induced by adding 2ug/ml of doxycycline for 24 or 48 hours. GFP-RNF152 is stably expressed in the cell. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
