Experiment Overview
| Repository ID: | FR-FCM-Z466 | Experiment name: | TCPP | MIFlowCyt score: | 81.21% |
| Primary researcher: | Lydia Bederka | PI/manager: | Vivienne Rebel | Uploaded by: | Lydia Bederka |
| Experiment dates: | 2018-09-04 - 2021-06-29 | Dataset uploaded: | Jun 2021 | Last updated: | Jul 2021 |
| Keywords: | [flow cytometry] [High Throughput] [Leukocytes] [macrophages] [sputum] [alveolar] [interstitial] [diagnostic] [lung cancer] [epithelial] | Manuscripts: |
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| Organizations: |
bioAffinity Technologies, bioAffinity Technologies, San Antonio, TX (United States)
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| Purpose: | Analysis of the cellular content of sputum via flow cytometry in order to develop a diagnostic platform for lung cancer. | ||||
| Conclusion: | Sputum-derived cells were stained with the porphyrin dye (TCPP) and several differences were revealed when comparing sputum from persons with confirmed lung cancer versus those at high-risk of developing lung cancer. | ||||
| Comments: | Three-day sputum collection from persons aged 55-74 with at least a 3- pack-year cigarette smoking history at high-risk of developing lung cancer. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Debris was omitted by defining the initial light scatter gate by using beads of known diameter. Particulates smaller than 5 um and larger than 30 um were omited from furhter analysis. Dead cells were excluded by the addition of a viability stain. The presence of Alveolar (CD45+ CD206++) and Interstitial macrophages (CD45+ CD206+) indicate the sample was derived from deep within the lung and not cells from the oral cavity. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · Compensation | A549 + 5X FVS-510.fcs · A549 + TCPP.fcs · A549 + perm + pan-CK.fcs · A549 unstained.fcs |
| · Unstained | BL-18-084 Unstained.fcs |
| · Isotype | BL-18-084 - Isotypes.fcs |
| · Experimental | BL-18-084 - Blood.fcs · BL-18-084 - Epithelial.fcs |
| · Beads | NiST Beads.fcs · Rainbow Beads.fcs |
