Experiment Overview
| Repository ID: | FR-FCM-Z46B | Experiment name: | FMO_Epithelial | MIFlowCyt score: | 81.45% |
| Primary researcher: | Lydia Bederka | PI/manager: | Vivienne Rebel | Uploaded by: | Lydia Bederka |
| Experiment dates: | 2021-03-26 - 2021-07-02 | Dataset uploaded: | Jul 2021 | Last updated: | Jul 2021 |
| Keywords: | [flow cytometry] [sputum] [diagnostic] [epithelial cells] [high] | Manuscripts: |
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| Organizations: |
bioAffinity Technologies, bioAffinity Technologies, San Antonio, TX (United States)
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| Purpose: | Identify main populations of sputum-derived cells by comparing the fluorescent profiles produced by isotype antibodies versus the fluorescent minus one (FMO) parameter. | ||||
| Conclusion: | FMO profiles produced more defined population boundaries when compared to isotype antibody profiles, allowing for cleaner cell population gate definitions. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | The LSR II cytometer platforms were checked prior to each session with BD FACSDiva CS&T Research beads. PMTs were adjusted and checked prior to each use using SPHERO Rainbow Calibration Particles. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · Control | 2021-03-26_ML_FMO_EPI_s_Epi_Control_1_015.fcs · 2021-03-26_ML_FMO_EPI_s_Epi_Control_2_016.fcs · 2021-03-26_ML_FMO_EPI_s_Epi_Control_3_017.fcs |
| · Isotype control | 2021-03-26_ML_FMO_EPI_s_Isotype_1_normal_011.fcs · 2021-03-26_ML_FMO_EPI_s_Isotype_2_TCPP_012.fcs · 2021-03-26_ML_FMO_EPI_s_Isotype_3_No FITC_013.fcs · 2021-03-26_ML_FMO_EPI_s_Isotype_4_No FITC_No TCPP_014.fcs |
| · Experimental | 2021-03-26_ML_FMO_EPI_s_Blood_018.fcs · 2021-03-26_ML_FMO_EPI_s_Epi_019.fcs |
| · Unstained | 2021-03-26_ML_FMO_EPI_s_Unstained_010.fcs |
