Experiment Overview
| Repository ID: | FR-FCM-Z4GQ | Experiment name: | Tracking cell cycling in RPE-Fucci cell cultures via DNA content profiling | MIFlowCyt score: | 94.00% |
| Primary researcher: | Qian Wang | PI/manager: | Manuel Goncalves | Uploaded by: | Qian Wang |
| Experiment dates: | 2021-06-24 - 2021-09-26 | Dataset uploaded: | Sep 2021 | Last updated: | Sep 2022 |
| Keywords: | [FUCCI-RPE] [Hoechst 33342 staining] | Manuscripts: | |||
| Organizations: |
Leiden University Medical Center, Cell and Chemical Biology, Leiden, South Holland (Netherlands)
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| Purpose: | Tracking cell cycling in RPE-Fucci cell cultures via DNA content profiling | ||||
| Conclusion: | During the cumulative tracing period, a clear serum concentration-dependent increase in the proportion of cells in S and G2/M was observed. | ||||
| Comments: | Flow cytometry assessments of DNA content profiles were performed at the indicated timepoints on cultures of RPE-Fucci cells treated with different FBS concentrations. Staining with the DNA dye Hoechst 33342 was performed to determine RPE-Fucci cell fractions at different stages of the cell cycle based on their DNA content. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Mock cells were used to set fluorescence background thresholds. | ||||
