Experiment Overview
| Repository ID: | FR-FCM-Z4GX | Experiment name: | Determining AdVP transduction efficiency in HeLa cells | MIFlowCyt score: | 93.09% |
| Primary researcher: | Qian Wang | PI/manager: | Manuel Goncalves | Uploaded by: | Qian Wang |
| Experiment dates: | 2021-06-15 - 2021-06-27 | Dataset uploaded: | Sep 2021 | Last updated: | Sep 2022 |
| Keywords: | [HeLa ] [AdVP.mCherry] | Manuscripts: | |||
| Organizations: |
Leiden University Medical Center, Cell and Chemical Biology, Leiden, South Holland (Netherlands)
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| Purpose: | Quantification of AdVP transduction levels in HeLa cells | ||||
| Conclusion: | Quantification of transduction levels showed that applying AdVPs at a multiplicity of infection (MOI) at or above 3000 vector genome copies per cell (GC cell-1) led to transgene expression in virtually all HeLa cells. | ||||
| Comments: | HeLa cells were treated with the indicated amounts of AdVP.mCherry. The frequencies of transduced cells and the protein expression levels per transduced cell (left and right graph, respectively), were assessed by mCherry-directed flow cytometry at 3 days post-transduction. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Mock-transduced cells were used to set fluorescence background thresholds. | ||||
