Experiment Overview
| Repository ID: | FR-FCM-Z4LE | Experiment name: | RCC | MIFlowCyt score: | 47.75% |
| Primary researcher: | Sara De Biasi | PI/manager: | Sara De Biasi | Uploaded by: | Sara De Biasi |
| Experiment dates: | 2017-02-01 - 2021-10-14 | Dataset uploaded: | Oct 2021 | Last updated: | Oct 2021 |
| Keywords: | [T cells] [anti-PD-1 therapy] [mRCC] [single-cells technologies] [unsupervised cluster analysis] | Manuscripts: | |||
| Organizations: |
University of Modena and Reggio Emilia, Modena, (Italy)
|
||||
| Purpose: | Renal-cell carcinoma (RCC) is responsible for the majority of tumors arising from the kidney parenchyma. Although a progressive improvement in median overall survival has been observed after the introduction of anti-PD-1 therapy, many patients do not benefit from this treatment. Therefore, we have investigated T cell dynamics to find immune modification induced by anti-PD-1 therapy. | ||||
| Conclusion: | Here, we show that, after therapy, RCC patients (5 responders and 15 non-responders) are characterized by a redistribution of different subsets across the memory T cell compartment. | ||||
| Comments: | In these patients, stem cell memory T cells (TSCM) and recently activated central memory T cell subset decrease. TSCM reservoir loses proliferative potential and is consumed during therapy, but increases the production of Th1 cytokines. In conclusion, in these RCC patients, anti-PD-1 therapy reshapes circulating memory T cells and induces a decrease of TSCM pool. | ||||
| Funding: | This work was funded by Fondazione Cassa di Risparmio di Modena to GP (2017); by Fondo di Ateneo per la Ricerca (FAR) 2017 to AC; by unrestricted grants to AC from: Sanfelice 1873 Banca Popolare (San Felice sul Panaro, Modena, Italy), Rotary CLUB Distretto 2072 (Modena, Modena L.A. Muratori, Carpi, Sassuolo, Ca-stelvetro di Modena, Italy). | ||||
| Quality control: | Interdonor comparison cPBMCs | ||||
