Experiment Overview
| Repository ID: | FR-FCM-Z4LG | Experiment name: | A 21-marker 18-color flow cytometry panel for in-depth phenotyping of human peripheral monocytes. | MIFlowCyt score: | 61.25% |
| Primary researcher: | Kathryn Hally | PI/manager: | Kathryn Hally | Uploaded by: | Kathryn Hally |
| Experiment dates: | 2019-12-01 - 2021-02-28 | Dataset uploaded: | Oct 2021 | Last updated: | Dec 2021 |
| Keywords: | [monocytes] [OMIP] [PBMCs] [innate immunity] [full spectrum flow cytometry] [human immunophenotyping] | Manuscripts: | |||
| Organizations: |
The University of Otago, Department of Surgery and Anaesthesia, Wellington, (New Zealand)
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| Purpose: | This 18-marker, 21-color panel has been designed to delineate and characterize four subsets of monocytes (classical, intermediate, SLAN- non-classical and SLAN+ non-classical) from peripheral human blood. | ||||
| Conclusion: | We successfully optimized an 18-marker, 21-color monocyte-centric human immunophenotyping panel. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Cytek CQ calibration beads were used for instrumental QC, performed at every run. The QC software adjusts laser delays, area scaling factors and gains settings to account for instrument variability over time. | ||||
