Experiment Overview
| Repository ID: | FR-FCM-Z4MS | Experiment name: | Immune cell phenotypes associated with disease severity and long-term neutralizing antibody titers after natural dengue virus infection | MIFlowCyt score: | 68.50% |
| Primary researcher: | Melissa Chng | PI/manager: | Melissa Chng | Uploaded by: | Melissa Chng |
| Experiment dates: | 2021-11-03 - | Dataset uploaded: | Nov 2021 | Last updated: | Nov 2021 |
| Keywords: | [T cell] [mass cytometry] [CyTOF] [Dengue] [DENV] [Tetramers] | Manuscripts: | [34095880] [PMC8149372] | ||
| Organizations: |
Agency for Science, Technology and Research, Singapore Immunology Network, Singapore, (Singapore)
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| Purpose: | Prior immunological exposure to dengue virus can be both protective and disease-enhancing during subse- quent infections with different dengue virus serotypes. We provide here a systematic, longitudinal analysis of B cell, T cell, and antibody responses in the same patients. | ||||
| Conclusion: | Antibody responses as well as T and B cell acti- vation differentiate primary from secondary responses. Hospitalization is associated with lower frequencies of activated, terminally differentiated T cells and higher percentages of effector memory CD4 T cells. Patients with more severe disease tend to have higher percentages of plasmablasts. This does not translate into long- term antibody titers, since neutralizing titers after 6 months correlate with percentages of specific memory B cells, but not with acute plasmablast activation. | ||||
| Comments: | None | ||||
| Funding: | The work was supported by an MRL/Singapore project grant (062015). K.F. and E.W.N. were supported by SIgN core funding. B.L. is part of the SIgN im- munomonitoring platform (supported by a BMRC IAF 311006 grant, BMRC transition funds #H16/99/b0/011, a BMRC IAF-PP H19/01/a0/024 SIGNAL grant, and an NRF SIS NRF2017_SISFP09 grant). | ||||
| Quality control: | The same control sample FMFG was used in all experiments. | ||||
Experiment variables
| Timepoints | |
|---|---|
| · _2 | 1_LNA001_2.fcs · 1_LNA006_2.fcs · 1_LNA013_2.fcs · 1_LNA016_2.fcs · 1_LNA025_2.fcs · 1_LNA031_2.fcs · 2_LNA011_2.fcs · 2_LNA038_2.fcs · 2_LNA040_2.fcs · 2_LNA051_2.fcs · 2_LNA052_2.fcs · 2_LNA063_2.fcs · 3_LNA004_2.fcs · 3_LNA005_2.fcs · 3_LNA010_2.fcs · 3_LNA012_2.fcs · 3_LNA029_2.fcs · 3_LNA041_2.fcs · 4_LNA009_2.fcs · 4_LNA015_2.fcs · 4_LNA046_2.fcs · 4_LNA047_2.fcs · 4_LNA059_2.fcs · 4_LNA070_2.fcs · 5_LNA030_2.fcs · 5_LNA034_2.fcs · 5_LNA036_2.fcs · 5_LNA048_2.fcs · 5_LNA058_2.fcs · 5_LNA060_2.fcs · 5_LNA061_2.fcs · 5_LNA065_2.fcs · 6_LNA014_2.fcs · 6_LNA018_2.fcs · 6_LNA019_2.fcs · 6_LNA023_2.fcs · 6_LNA032_2.fcs · 6_LNA033_2.fcs · 6_LNA043_2.fcs · 6_LNA062_2.fcs |
| · _3 | 1_LNA001_3.fcs · 1_LNA006_3.fcs · 1_LNA013_3.fcs · 1_LNA016_3.fcs · 1_LNA025_3.fcs · 1_LNA031_3.fcs · 2_LNA011_3.fcs · 2_LNA038_3.fcs · 2_LNA040_3.fcs · 2_LNA051_3.fcs · 2_LNA052_3.fcs · 2_LNA063_3.fcs · 3_LNA004_3.fcs · 3_LNA005_3.fcs · 3_LNA010_3.fcs · 3_LNA012_3.fcs · 3_LNA029_3.fcs · 3_LNA041_3.fcs · 4_LNA009_3.fcs · 4_LNA015_3.fcs · 4_LNA047_3.fcs · 4_LNA059_3.fcs · 5_LNA030_3.fcs · 5_LNA048_3.fcs · 5_LNA060_3.fcs · 5_LNA061_3.fcs · 5_LNA065_3.fcs · 6_LNA014_3.fcs · 6_LNA018_3.fcs · 6_LNA019_3.fcs · 6_LNA023_3.fcs · 6_LNA032_3.fcs · 6_LNA043_3.fcs · 6_LNA062_3.fcs |
| · _4 | 1_LNA001_4.fcs · 1_LNA006_4.fcs · 1_LNA013_4.fcs · 1_LNA016_4.fcs · 1_LNA025_4.fcs · 1_LNA031_4.fcs · 2_LNA011_4.fcs · 2_LNA038_4.fcs · 2_LNA040_4.fcs · 2_LNA051_4.fcs · 2_LNA052_4.fcs · 2_LNA063_4.fcs · 3_LNA002_4.fcs · 3_LNA005_4.fcs · 3_LNA010_4.fcs · 3_LNA012_4.fcs · 3_LNA029_4.fcs · 4_LNA009_4.fcs · 4_LNA047_4.fcs · 4_LNA059_4.fcs · 5_LNA030_4.fcs · 5_LNA048_4.fcs · 5_LNA060_4.fcs · 5_LNA061_4.fcs · 5_LNA065_4.fcs · 6_LNA014_4.fcs · 6_LNA018_4.fcs · 6_LNA019_4.fcs · 6_LNA023_4.fcs · 6_LNA032_4.fcs · 6_LNA062_4.fcs |
| · _5 | 1_LNA001_5.fcs · 1_LNA006_5.fcs · 1_LNA013_5.fcs · 1_LNA016_5.fcs · 1_LNA025_5.fcs · 1_LNA031_5.fcs · 2_LNA038_5.fcs · 2_LNA040_5.fcs · 2_LNA051_5.fcs · 2_LNA052_5.fcs · 2_LNA063_5.fcs · 3_LNA004_5.fcs · 3_LNA005_5.fcs · 3_LNA010_5.fcs · 3_LNA012_5.fcs · 3_LNA029_5.fcs · 4_LNA009_5.fcs · 4_LNA047_5.fcs · 5_LNA030_5.fcs · 5_LNA048_5.fcs · 5_LNA060_5.fcs · 5_LNA061_5.fcs · 6_LNA014_5.fcs · 6_LNA018_5.fcs · 6_LNA019_5.fcs · 6_LNA032_5.fcs · 6_LNA033_5.fcs · 6_LNA048_5.fcs |
| · _0 | 2_AV9G_0.fcs · 3_AUX6_0.fcs · 3_FMFG_0.fcs · 6_9D4J_0.fcs |
| · _1 | 6_LNA048_1.fcs |
| Individuals | |
|---|---|
| · LNA001 | 1_LNA001_2.fcs · 1_LNA001_3.fcs · 1_LNA001_4.fcs · 1_LNA001_5.fcs |
| · LNA006 | 1_LNA006_2.fcs · 1_LNA006_3.fcs · 1_LNA006_4.fcs · 1_LNA006_5.fcs |
| · LNA013 | 1_LNA013_2.fcs · 1_LNA013_3.fcs · 1_LNA013_4.fcs · 1_LNA013_5.fcs |
| · LNA016 | 1_LNA016_2.fcs · 1_LNA016_3.fcs · 1_LNA016_4.fcs · 1_LNA016_5.fcs |
| · LNA025 | 1_LNA025_2.fcs · 1_LNA025_3.fcs · 1_LNA025_4.fcs · 1_LNA025_5.fcs |
| · LNA031 | 1_LNA031_2.fcs · 1_LNA031_3.fcs · 1_LNA031_4.fcs · 1_LNA031_5.fcs |
| · AV9G | 2_AV9G_0.fcs |
| · LNA011 | 2_LNA011_2.fcs · 2_LNA011_3.fcs · 2_LNA011_4.fcs |
| · LNA038 | 2_LNA038_2.fcs · 2_LNA038_3.fcs · 2_LNA038_4.fcs · 2_LNA038_5.fcs |
| · LNA040 | 2_LNA040_2.fcs · 2_LNA040_3.fcs · 2_LNA040_4.fcs · 2_LNA040_5.fcs |
| · LNA051 | 2_LNA051_2.fcs · 2_LNA051_3.fcs · 2_LNA051_4.fcs · 2_LNA051_5.fcs |
| · LNA052 | 2_LNA052_2.fcs · 2_LNA052_3.fcs · 2_LNA052_4.fcs · 2_LNA052_5.fcs |
| · LNA063 | 2_LNA063_2.fcs · 2_LNA063_3.fcs · 2_LNA063_4.fcs · 2_LNA063_5.fcs |
| · AUX6 | 3_AUX6_0.fcs |
| · FMFG | 3_FMFG_0.fcs |
| · LNA002 | 3_LNA002_4.fcs |
| · LNA004 | 3_LNA004_2.fcs · 3_LNA004_3.fcs · 3_LNA004_5.fcs |
| · LNA005 | 3_LNA005_2.fcs · 3_LNA005_3.fcs · 3_LNA005_4.fcs · 3_LNA005_5.fcs |
| · LNA010 | 3_LNA010_2.fcs · 3_LNA010_3.fcs · 3_LNA010_4.fcs · 3_LNA010_5.fcs |
| · LNA012 | 3_LNA012_2.fcs · 3_LNA012_3.fcs · 3_LNA012_4.fcs · 3_LNA012_5.fcs |
| · LNA029 | 3_LNA029_2.fcs · 3_LNA029_3.fcs · 3_LNA029_4.fcs · 3_LNA029_5.fcs |
| · LNA041 | 3_LNA041_2.fcs · 3_LNA041_3.fcs |
| · LNA009 | 4_LNA009_2.fcs · 4_LNA009_3.fcs · 4_LNA009_4.fcs · 4_LNA009_5.fcs |
| · LNA015 | 4_LNA015_2.fcs · 4_LNA015_3.fcs |
| · LNA046 | 4_LNA046_2.fcs |
| · LNA047 | 4_LNA047_2.fcs · 4_LNA047_3.fcs · 4_LNA047_4.fcs · 4_LNA047_5.fcs |
| · LNA059 | 4_LNA059_2.fcs · 4_LNA059_3.fcs · 4_LNA059_4.fcs |
| · LNA070 | 4_LNA070_2.fcs |
| · LNA030 | 5_LNA030_2.fcs · 5_LNA030_3.fcs · 5_LNA030_4.fcs · 5_LNA030_5.fcs |
| · LNA034 | 5_LNA034_2.fcs |
| · LNA036 | 5_LNA036_2.fcs |
| · LNA048 | 5_LNA048_2.fcs · 5_LNA048_3.fcs · 5_LNA048_4.fcs · 5_LNA048_5.fcs · 6_LNA048_1.fcs · 6_LNA048_5.fcs |
| · LNA058 | 5_LNA058_2.fcs |
| · LNA060 | 5_LNA060_2.fcs · 5_LNA060_3.fcs · 5_LNA060_4.fcs · 5_LNA060_5.fcs |
| · LNA061 | 5_LNA061_2.fcs · 5_LNA061_3.fcs · 5_LNA061_4.fcs · 5_LNA061_5.fcs |
| · LNA065 | 5_LNA065_2.fcs · 5_LNA065_3.fcs · 5_LNA065_4.fcs |
| · 9D4J | 6_9D4J_0.fcs |
| · LNA014 | 6_LNA014_2.fcs · 6_LNA014_3.fcs · 6_LNA014_4.fcs · 6_LNA014_5.fcs |
| · LNA018 | 6_LNA018_2.fcs · 6_LNA018_3.fcs · 6_LNA018_4.fcs · 6_LNA018_5.fcs |
| · LNA019 | 6_LNA019_2.fcs · 6_LNA019_3.fcs · 6_LNA019_4.fcs · 6_LNA019_5.fcs |
| · LNA023 | 6_LNA023_2.fcs · 6_LNA023_3.fcs · 6_LNA023_4.fcs |
| · LNA032 | 6_LNA032_2.fcs · 6_LNA032_3.fcs · 6_LNA032_4.fcs · 6_LNA032_5.fcs |
| · LNA033 | 6_LNA033_2.fcs · 6_LNA033_5.fcs |
| · LNA043 | 6_LNA043_2.fcs · 6_LNA043_3.fcs |
| · LNA062 | 6_LNA062_2.fcs · 6_LNA062_3.fcs · 6_LNA062_4.fcs |
