Experiment Overview
| Repository ID: | FR-FCM-Z4NB | Experiment name: | A 33-color panel for phenotypic analysis of murine organ specific immune cells | MIFlowCyt score: | 79.25% |
| Primary researcher: | Siyu Yang | PI/manager: | Siyu Yang | Uploaded by: | Siyu Yang |
| Experiment dates: | 2020-12-20 - 2021-07-20 | Dataset uploaded: | Nov 2021 | Last updated: | Nov 2021 |
| Keywords: | [flow cytometry] [33-color panel] [phenotypic analysis] [organ specific immune cells] | Manuscripts: | |||
| Organizations: |
Guangdong Provincial People’s Hospital, Guangzhou, (China)
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| Purpose: | This 33-color panel delineates immune phenotypes of the major lymphocyte subsets in murine tissues | ||||
| Conclusion: | The 33-color panel using Full Spectrum Flow Cytometry identified major subpopulations of lymphocytes in mouse organs with good resolution. This panel will be useful to identify and quantify the major lymphocytes populations for different mouse organs in autoimmune diseases or cancer. | ||||
| Comments: | The 33-color panel using Full Spectrum Flow Cytometry identified major subpopulations of lymphocytes in mouse organs with good resolution. This panel will be useful to identify and quantify the major lymphocytes populations for different mouse organs in autoimmune diseases or cancer. | ||||
| Funding: | This work was supported by Program for Guangdong Introducing Innovative and Entrepreneurial Teams (2017ZT07S054), National Natural Science Foundation of China (81901652), and State Key Laboratory of Pathogenesis, Prevention, Treatment of Central Asian High Incidence Diseases Fund, China (SKL-HIDCA-2021-7). | ||||
| Quality control: | Daily QC was performed using SpectroFlo® QC beads prior to acquiring samples to ensure that the cytometer was performing optimally, Antibody titrations for selection of optimal titers to reduce spillover spreading error, FMO stain controls for some weakly expressed antigens to exclude the influence from other fluorochromes. | ||||
