Experiment Overview
| Repository ID: | FR-FCM-Z4NC | Experiment name: | Figure 4A/S2A/S2B | MIFlowCyt score: | 73.75% |
| Primary researcher: | Raffaele Altamura | PI/manager: | Raffaele Altamura | Uploaded by: | Raffaele Altamura |
| Experiment dates: | 2017-11-10 - 2017-11-30 | Dataset uploaded: | Nov 2021 | Last updated: | Nov 2021 |
| Keywords: | [Codon optimization] [coding sequence diversification] | Manuscripts: | |||
| Organizations: |
ETH Zurich, Zurich, (Switzerland)
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| Purpose: | Testing the expression strength of mCitrine coding sequence variants in CHO cells | ||||
| Conclusion: | A set of mCitrine coding sequences designed with a custom-made sequence diversifier software tool outperforms sequences were codon have been randomly assigned | ||||
| Comments: | Raw data for manuscript figures 4A, S2A, S2B | ||||
| Funding: | ETH Zurich, NCCR Molecular Systems Engineering (Switzerland) | ||||
| Quality control: | All transfections and subsequent flow cytometric analyses were performed in triplicates. A negative control (untrasfected) was included. A transfection control marker (mCherry) was included for normalization. | ||||
