Experiment Overview
| Repository ID: | FR-FCM-Z4PL | Experiment name: | Cutaneous Lupus Erythematosus and Healthy Donor Blister Fluid and Blood Analysis - Subject #1 | MIFlowCyt score: | 63.25% |
| Primary researcher: | Jillian Richmond | PI/manager: | Jillian Richmond | Uploaded by: | Jillian Richmond |
| Experiment dates: | 2019-02-07 - | Dataset uploaded: | Dec 2021 | Last updated: | Oct 2023 |
| Keywords: | [Blood] [healthy] [Skin] [lupus] [CXCR3] [CD38] | Manuscripts: | |||
| Organizations: |
UMass Chan Medical School, Dermatology, Worcester, MA (USA)
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| Purpose: | To analyze skin infiltrating immune cells in blister fluid compared to blood, and to assess CXCR3 and CD38 expression on these cells in CLE and healthy donors. | ||||
| Conclusion: | Please refer to associated publications for conclusions. | ||||
| Comments: | Please refer to .wsp and .xlsx files for gating and normalized counts, respectively. | ||||
| Funding: | Lupus Research Alliance - Target Identification in Lupus Award Dermatology Foundation - Women's Health Career Development Award | ||||
| Quality control: | None | ||||
Experiment variables
| Doses | |
|---|---|
| · 5ul/test | APC (Beads).fcs · APC-Cy7 (Beads).fcs · Alexa Fluor 647 (Beads).fcs · Alexa Fluor 700 (Beads).fcs · BV421 (Beads).fcs · BV570 (Beads).fcs · BV605 (Beads).fcs · BV650 (Beads).fcs · BV711 (Beads).fcs · BV785 (Beads).fcs · FITC (Beads).fcs · Ghost Dye BV510 (Beads).fcs · PE (Beads).fcs · PE-Cy7 (Beads).fcs · PE-Dazzle594 (Beads).fcs · Pacific Blue (Beads).fcs · PerCP-Cy5.5 (Beads).fcs · Unstained (Cells).fcs · APC FMO.fcs · BV570 FMO.fcs · BV605 FMO.fcs · BV711 FMO.fcs · Lesion 2.fcs · PE FMO.fcs · PacBlue FMO.fcs · blood.fcs · nonlesional.fcs |
| Timepoints | |
|---|---|
| · N/A | APC (Beads).fcs · APC-Cy7 (Beads).fcs · Alexa Fluor 647 (Beads).fcs · Alexa Fluor 700 (Beads).fcs · BV421 (Beads).fcs · BV570 (Beads).fcs · BV605 (Beads).fcs · BV650 (Beads).fcs · BV711 (Beads).fcs · BV785 (Beads).fcs · FITC (Beads).fcs · Ghost Dye BV510 (Beads).fcs · PE (Beads).fcs · PE-Cy7 (Beads).fcs · PE-Dazzle594 (Beads).fcs · Pacific Blue (Beads).fcs · PerCP-Cy5.5 (Beads).fcs · Unstained (Cells).fcs · APC FMO.fcs · BV570 FMO.fcs · BV605 FMO.fcs · BV711 FMO.fcs · Lesion 2.fcs · PE FMO.fcs · PacBlue FMO.fcs · blood.fcs · nonlesional.fcs |
| Conditions | |
|---|---|
| · Lupus | Unstained (Cells).fcs · Lesion 2.fcs · blood.fcs · nonlesional.fcs |
| · N/A | APC (Beads).fcs · APC-Cy7 (Beads).fcs · Alexa Fluor 647 (Beads).fcs · Alexa Fluor 700 (Beads).fcs · BV421 (Beads).fcs · BV570 (Beads).fcs · BV605 (Beads).fcs · BV650 (Beads).fcs · BV711 (Beads).fcs · BV785 (Beads).fcs · FITC (Beads).fcs · Ghost Dye BV510 (Beads).fcs · PE (Beads).fcs · PE-Cy7 (Beads).fcs · PE-Dazzle594 (Beads).fcs · Pacific Blue (Beads).fcs · PerCP-Cy5.5 (Beads).fcs · APC FMO.fcs · BV570 FMO.fcs · BV605 FMO.fcs · BV711 FMO.fcs · PE FMO.fcs · PacBlue FMO.fcs |
| Individuals | |
|---|---|
| · Subject 1 | Lesion 2.fcs · blood.fcs · nonlesional.fcs |
| · N/A | APC (Beads).fcs · APC-Cy7 (Beads).fcs · Alexa Fluor 647 (Beads).fcs · Alexa Fluor 700 (Beads).fcs · BV421 (Beads).fcs · BV570 (Beads).fcs · BV605 (Beads).fcs · BV650 (Beads).fcs · BV711 (Beads).fcs · BV785 (Beads).fcs · FITC (Beads).fcs · Ghost Dye BV510 (Beads).fcs · PE (Beads).fcs · PE-Cy7 (Beads).fcs · PE-Dazzle594 (Beads).fcs · Pacific Blue (Beads).fcs · PerCP-Cy5.5 (Beads).fcs · Unstained (Cells).fcs · APC FMO.fcs · BV570 FMO.fcs · BV605 FMO.fcs · BV711 FMO.fcs · PE FMO.fcs · PacBlue FMO.fcs |
| Sample Type | |
|---|---|
| · Blood | blood.fcs |
| · Blister fluid | Lesion 2.fcs · nonlesional.fcs |
| · Beads | APC (Beads).fcs · APC-Cy7 (Beads).fcs · Alexa Fluor 647 (Beads).fcs · Alexa Fluor 700 (Beads).fcs · BV421 (Beads).fcs · BV570 (Beads).fcs · BV605 (Beads).fcs · BV650 (Beads).fcs · BV711 (Beads).fcs · BV785 (Beads).fcs · FITC (Beads).fcs · Ghost Dye BV510 (Beads).fcs · PE (Beads).fcs · PE-Cy7 (Beads).fcs · PE-Dazzle594 (Beads).fcs · Pacific Blue (Beads).fcs · PerCP-Cy5.5 (Beads).fcs |
| · FMOs | Unstained (Cells).fcs · APC FMO.fcs · BV570 FMO.fcs · BV605 FMO.fcs · BV711 FMO.fcs · PE FMO.fcs · PacBlue FMO.fcs |
