Experiment Overview
| Repository ID: | FR-FCM-Z4PM | Experiment name: | Cutaneous Lupus Erythematosus and Healthy Donor Blister Fluid and Blood Analysis - Subject #2 | MIFlowCyt score: | 63.25% |
| Primary researcher: | Jillian Richmond | PI/manager: | Jillian Richmond | Uploaded by: | Jillian Richmond |
| Experiment dates: | 2018-12-17 - | Dataset uploaded: | Dec 2021 | Last updated: | Oct 2023 |
| Keywords: | [Blood] [healthy] [lupus] [CXCR3] [CD38] | Manuscripts: | |||
| Organizations: |
UMass Chan Medical School, Dermatology, Worcester, MA (United States)
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| Purpose: | To analyze skin infiltrating immune cells in blister fluid compared to blood, and to assess CXCR3 and CD38 expression on these cells in CLE and healthy donors. | ||||
| Conclusion: | Please see associated publications | ||||
| Comments: | Please refer to supplied .wsp files and .xlsx files for gating and normalization respectively. | ||||
| Funding: | Lupus Research Alliance - Target Identification in Lupus Award Dermatology Foundation - Women's Health Career Development Award | ||||
| Quality control: | None | ||||
Experiment variables
| Doses | |
|---|---|
| · 5ul/test | CXCR3 FMO_20181217_163726_Unmixed.fcs · Reference Group_APC (Beads)_20181217_161902_Unmixed.fcs · Reference Group_APC-Cy7 (Beads)_20181217_162123_Unmixed.fcs · Reference Group_Alexa Fluor 647 (Beads)_20181217_161948_Unmixed.fcs · Reference Group_Alexa Fluor 700 (Beads)_20181217_162037_Unmixed.fcs · Reference Group_BV421 (Beads)_20181217_160753_Unmixed.fcs · Reference Group_BV570 (Beads)_20181217_161053_Unmixed.fcs · Reference Group_BV605 (Beads)_20181217_161145_Unmixed.fcs · Reference Group_BV650 (Beads)_20181217_161239_Unmixed.fcs · Reference Group_BV711 (Beads)_20181217_161330_Unmixed.fcs · Reference Group_BV785 (Beads)_20181217_161416_Unmixed.fcs · Reference Group_FITC (Beads)_20181217_161459_Unmixed.fcs · Reference Group_Ghost Dye BV510 (Beads)_20181217_160956_Unmixed.fcs · Reference Group_PE (Beads)_20181217_161557_Unmixed.fcs · Reference Group_PE-Cy7 (Beads)_20181217_161815_Unmixed.fcs · Reference Group_PE-Dazzle594 (Beads)_20181217_161646_Unmixed.fcs · Reference Group_Pacific Blue (Beads)_20181217_160841_Unmixed.fcs · Reference Group_PerCP-Cy5.5 (Beads)_20181217_161725_Unmixed.fcs · Reference Group_Unstained (Beads)_20181217_160707_Unmixed.fcs · blood_20181217_163301_Unmixed.fcs · lesional blister_20181217_162714_Unmixed.fcs · nonlesional blister_20181217_162916_Unmixed.fcs |
| Timepoints | |
|---|---|
| · N/A | CXCR3 FMO_20181217_163726_Unmixed.fcs · Reference Group_APC (Beads)_20181217_161902_Unmixed.fcs · Reference Group_APC-Cy7 (Beads)_20181217_162123_Unmixed.fcs · Reference Group_Alexa Fluor 647 (Beads)_20181217_161948_Unmixed.fcs · Reference Group_Alexa Fluor 700 (Beads)_20181217_162037_Unmixed.fcs · Reference Group_BV421 (Beads)_20181217_160753_Unmixed.fcs · Reference Group_BV570 (Beads)_20181217_161053_Unmixed.fcs · Reference Group_BV605 (Beads)_20181217_161145_Unmixed.fcs · Reference Group_BV650 (Beads)_20181217_161239_Unmixed.fcs · Reference Group_BV711 (Beads)_20181217_161330_Unmixed.fcs · Reference Group_BV785 (Beads)_20181217_161416_Unmixed.fcs · Reference Group_FITC (Beads)_20181217_161459_Unmixed.fcs · Reference Group_Ghost Dye BV510 (Beads)_20181217_160956_Unmixed.fcs · Reference Group_PE (Beads)_20181217_161557_Unmixed.fcs · Reference Group_PE-Cy7 (Beads)_20181217_161815_Unmixed.fcs · Reference Group_PE-Dazzle594 (Beads)_20181217_161646_Unmixed.fcs · Reference Group_Pacific Blue (Beads)_20181217_160841_Unmixed.fcs · Reference Group_PerCP-Cy5.5 (Beads)_20181217_161725_Unmixed.fcs · Reference Group_Unstained (Beads)_20181217_160707_Unmixed.fcs · blood_20181217_163301_Unmixed.fcs · lesional blister_20181217_162714_Unmixed.fcs · nonlesional blister_20181217_162916_Unmixed.fcs |
| Conditions | |
|---|---|
| · Samples | blood_20181217_163301_Unmixed.fcs · lesional blister_20181217_162714_Unmixed.fcs · nonlesional blister_20181217_162916_Unmixed.fcs |
| · Staining controls | CXCR3 FMO_20181217_163726_Unmixed.fcs · Reference Group_APC (Beads)_20181217_161902_Unmixed.fcs · Reference Group_APC-Cy7 (Beads)_20181217_162123_Unmixed.fcs · Reference Group_Alexa Fluor 647 (Beads)_20181217_161948_Unmixed.fcs · Reference Group_Alexa Fluor 700 (Beads)_20181217_162037_Unmixed.fcs · Reference Group_BV421 (Beads)_20181217_160753_Unmixed.fcs · Reference Group_BV570 (Beads)_20181217_161053_Unmixed.fcs · Reference Group_BV605 (Beads)_20181217_161145_Unmixed.fcs · Reference Group_BV650 (Beads)_20181217_161239_Unmixed.fcs · Reference Group_BV711 (Beads)_20181217_161330_Unmixed.fcs · Reference Group_BV785 (Beads)_20181217_161416_Unmixed.fcs · Reference Group_FITC (Beads)_20181217_161459_Unmixed.fcs · Reference Group_Ghost Dye BV510 (Beads)_20181217_160956_Unmixed.fcs · Reference Group_PE (Beads)_20181217_161557_Unmixed.fcs · Reference Group_PE-Cy7 (Beads)_20181217_161815_Unmixed.fcs · Reference Group_PE-Dazzle594 (Beads)_20181217_161646_Unmixed.fcs · Reference Group_Pacific Blue (Beads)_20181217_160841_Unmixed.fcs · Reference Group_PerCP-Cy5.5 (Beads)_20181217_161725_Unmixed.fcs · Reference Group_Unstained (Beads)_20181217_160707_Unmixed.fcs |
| Individuals | |
|---|---|
| · Subject 2 | blood_20181217_163301_Unmixed.fcs · lesional blister_20181217_162714_Unmixed.fcs · nonlesional blister_20181217_162916_Unmixed.fcs |
| · N/A | CXCR3 FMO_20181217_163726_Unmixed.fcs · Reference Group_APC (Beads)_20181217_161902_Unmixed.fcs · Reference Group_APC-Cy7 (Beads)_20181217_162123_Unmixed.fcs · Reference Group_Alexa Fluor 647 (Beads)_20181217_161948_Unmixed.fcs · Reference Group_Alexa Fluor 700 (Beads)_20181217_162037_Unmixed.fcs · Reference Group_BV421 (Beads)_20181217_160753_Unmixed.fcs · Reference Group_BV570 (Beads)_20181217_161053_Unmixed.fcs · Reference Group_BV605 (Beads)_20181217_161145_Unmixed.fcs · Reference Group_BV650 (Beads)_20181217_161239_Unmixed.fcs · Reference Group_BV711 (Beads)_20181217_161330_Unmixed.fcs · Reference Group_BV785 (Beads)_20181217_161416_Unmixed.fcs · Reference Group_FITC (Beads)_20181217_161459_Unmixed.fcs · Reference Group_Ghost Dye BV510 (Beads)_20181217_160956_Unmixed.fcs · Reference Group_PE (Beads)_20181217_161557_Unmixed.fcs · Reference Group_PE-Cy7 (Beads)_20181217_161815_Unmixed.fcs · Reference Group_PE-Dazzle594 (Beads)_20181217_161646_Unmixed.fcs · Reference Group_Pacific Blue (Beads)_20181217_160841_Unmixed.fcs · Reference Group_PerCP-Cy5.5 (Beads)_20181217_161725_Unmixed.fcs · Reference Group_Unstained (Beads)_20181217_160707_Unmixed.fcs |
| Sample Type | |
|---|---|
| · Blister fluid | lesional blister_20181217_162714_Unmixed.fcs · nonlesional blister_20181217_162916_Unmixed.fcs |
| · Blood | blood_20181217_163301_Unmixed.fcs |
| · Beads | Reference Group_APC (Beads)_20181217_161902_Unmixed.fcs · Reference Group_APC-Cy7 (Beads)_20181217_162123_Unmixed.fcs · Reference Group_Alexa Fluor 647 (Beads)_20181217_161948_Unmixed.fcs · Reference Group_Alexa Fluor 700 (Beads)_20181217_162037_Unmixed.fcs · Reference Group_BV421 (Beads)_20181217_160753_Unmixed.fcs · Reference Group_BV570 (Beads)_20181217_161053_Unmixed.fcs · Reference Group_BV605 (Beads)_20181217_161145_Unmixed.fcs · Reference Group_BV650 (Beads)_20181217_161239_Unmixed.fcs · Reference Group_BV711 (Beads)_20181217_161330_Unmixed.fcs · Reference Group_BV785 (Beads)_20181217_161416_Unmixed.fcs · Reference Group_FITC (Beads)_20181217_161459_Unmixed.fcs · Reference Group_Ghost Dye BV510 (Beads)_20181217_160956_Unmixed.fcs · Reference Group_PE (Beads)_20181217_161557_Unmixed.fcs · Reference Group_PE-Cy7 (Beads)_20181217_161815_Unmixed.fcs · Reference Group_PE-Dazzle594 (Beads)_20181217_161646_Unmixed.fcs · Reference Group_Pacific Blue (Beads)_20181217_160841_Unmixed.fcs · Reference Group_PerCP-Cy5.5 (Beads)_20181217_161725_Unmixed.fcs · Reference Group_Unstained (Beads)_20181217_160707_Unmixed.fcs |
| · FMOs | CXCR3 FMO_20181217_163726_Unmixed.fcs |
