Experiment Overview
| Repository ID: | FR-FCM-Z4RW | Experiment name: | Isolation of post-replicative male germ cells from adult mouse testes | MIFlowCyt score: | 93.50% |
| Primary researcher: | Mark Gill | PI/manager: | Antoine Peters | Uploaded by: | Mark Gill |
| Experiment dates: | 2019-05-08 - 2019-11-21 | Dataset uploaded: | Jan 2022 | Last updated: | Jan 2022 |
| Keywords: | [germ cells] [spermatogenesis] [spermatids] [primary spermatocytes] | Manuscripts: | |||
| Organizations: |
Friedrich Miescher Institute for Biomedical Research, Basel, (Switzerland)
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| Purpose: | To purify populations of post-replicative male germ cells from adult C57BL/6 testes by dual color DNA staining with Hoechst 33342 and SYTO 16. | ||||
| Conclusion: | 5 distinct populations of cells were isolated to high levels of purity from the testes of a single 3 month old C57BL/6 mouse. | ||||
| Comments: | None | ||||
| Funding: | SNF Project Grants #31003A-146293 and #31003A-172873 to Antoine H.F.M. Peters | ||||
| Quality control: | Isolated cells were fixed onto microscope slides and immuno-stained for markers to validate the identity of these cells | ||||
