Experiment Overview
| Repository ID: | FR-FCM-Z4U8 | Experiment name: | Figure 2 Part 3 | MIFlowCyt score: | 31.92% |
| Primary researcher: | Román Ramos Báez | PI/manager: | Jennifer Nemhauser | Uploaded by: | Román Ramos Báez |
| Experiment dates: | 2022-01-13 - 2022-01-13 | Dataset uploaded: | Jan 2022 | Last updated: | Sep 2022 |
| Keywords: | [fluorescence] [Saccharomyces cerevisiae] [transcription activity] [auxin] [TPL] | Manuscripts: | |||
| Organizations: |
University of Washington, Biology, Seattle, WA (United States)
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| Purpose: | This looks at the repressive function of Helix 1 of LisHs across eukaryotes. This is a continuation of the expriment. | ||||
| Conclusion: | We find that repressive function is well conserved across eukaryotes. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | We have a negative control (no repression alpha helix control, and no repression noIAA3 and no H1 control) and a positive control (strong repressor TPL H1).] We gate for live haploid yeast cells. We dilute in the morning and wait to log growth to make fluorescence measurements. | ||||
