Experiment Overview
| Repository ID: | FR-FCM-Z4U9 | Experiment name: | Unpublished Experiment 6 H1 Multimers | MIFlowCyt score: | 31.50% |
| Primary researcher: | Román Ramos Báez | PI/manager: | Jennifer Nemhauser | Uploaded by: | Román Ramos Báez |
| Experiment dates: | 2022-01-13 - 2022-01-13 | Dataset uploaded: | Jan 2022 | Last updated: | Sep 2022 |
| Keywords: | [fluorescence] [Saccharomyces cerevisiae] [transcription activity] [auxin] [TPL] | Manuscripts: | |||
| Organizations: |
University of Washington, Biology, Seattle, WA (United States)
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| Purpose: | We test the effects on the repressive function of having multimers of TPL LisH H1. We also test if having an unintegrated reporter, which should be stronger in fluorescence, can better show repression. | ||||
| Conclusion: | We find that multimers cannot repress as well as the H1 monomer. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | We have a negative control (no repression alpha helix control, and no repression noIAA3 and no H1 control) and a positive control (strong repressor TPL H1). We gate for live haploid yeast cells. We dilute in the morning and wait to log growth to make fluorescence measurements. | ||||
