Experiment Overview
| Repository ID: | FR-FCM-Z4UB | Experiment name: | Figure 1 TPL R6 | MIFlowCyt score: | 31.50% |
| Primary researcher: | Román Ramos Báez | PI/manager: | Jennifer Nemhauser | Uploaded by: | Román Ramos Báez |
| Experiment dates: | 2022-01-19 - 2022-01-19 | Dataset uploaded: | Jan 2022 | Last updated: | Sep 2022 |
| Keywords: | [fluorescence] [Saccharomyces cerevisiae] [transcription activity] [auxin] [TPL] | Manuscripts: | |||
| Organizations: |
University of Washington, Biology, Seattle, WA (United States)
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| Purpose: | We do site-saturation mutagenesis to look at the repressive function of residueR6 in H1 of LisH domain in TPL. We want to understand more about the role of this residue in inducible de-repressoin of auxin responsive transcription. | ||||
| Conclusion: | we find most mutations here do not interfere with repressive ability. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | We have a negative control (no repression alpha helix control, and no repression noIAA3 and no H1 control) and a positive control (strong repressor TPL H1). We gate for live haploid yeast cells. We dilute in the morning and wait to log growth to make fluorescence measurements. | ||||
