Experiment Overview
| Repository ID: | FR-FCM-Z4UN | Experiment name: | Supplemental figure3: REP, CTL:AIM-V, ICM, TAC | MIFlowCyt score: | 41.00% |
| Primary researcher: | Sarah MacPherson | PI/manager: | Julian Lum | Uploaded by: | Sarah MacPherson |
| Experiment dates: | 2021-12-03 - 2021-12-03 | Dataset uploaded: | Jan 2022 | Last updated: | Feb 2022 |
| Keywords: | None | Manuscripts: | |||
| Organizations: |
Trev & Joyce Deeley Research Centre, BC Cancer – Victoria, Victoria, BC (Canada)
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| Purpose: | Investigate the influence of activation versus media on function (TNFa) and mitochondrial mass (mitotracker green) on day 14 of expansion. REP was compared to CA5(CTL:AIM-V) icm stim and CA5 tac stim for the comparison of different activation methods within the same media. CA5, ICM and TAC media all stimulated with icm was a comparison of the influence of media. This was repeated (CA5, ICM and TAC media) but with tac stimulation. icm- Stemcell technologies CD3/CD28 activators tac - Miltenyi TransACT CD3/CD28 activators | ||||
| Conclusion: | None | ||||
| Comments: | The mitochondrial mass and function(TNFa) of the T cell product was influenced by the media. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
