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Experiment Overview

Repository ID: FR-FCM-Z4V4 Experiment name: Assessing Function of T cell products in Ovarian cancer ascites fluid MIFlowCyt score: 42.50%
Primary researcher: Sarah MacPherson PI/manager: Julian Lum Uploaded by: Sarah MacPherson
Experiment dates: 2021-03-12 - Dataset uploaded: Jan 2022 Last updated: Feb 2022
Keywords: None Manuscripts:
Organizations: Trev & Joyce Deeley Research Centre, BC Cancer – Victoria, Victoria, BC (Canada)
Purpose: To investigate how T cell products function in an immune suppressive environment by using ovarian cancer ascites fluid (IROC138). After 12 days of expansion, T cells were activated with plate bound CD3, soluble CD28 in ascites or media for 48 hours and function and mitochondrial activity was assessed.
Conclusion: All expansion conditions (REP, ICM, TAC, CMM, HPLM) were supressed in mitochondiral acitivty and function but to varying degrees.
Comments: None
Funding: Not disclosed
Quality control: None
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