Experiment Overview
| Repository ID: | FR-FCM-Z4VF | Experiment name: | B. subtilis yxjA RIboswitch: Broken Central Helix Mutants and Terminator Controls | MIFlowCyt score: | 29.25% |
| Primary researcher: | Luyi Cheng | PI/manager: | Julius Lucks | Uploaded by: | Luyi Cheng |
| Experiment dates: | 2021-01-25 - 2021-01-31 | Dataset uploaded: | Feb 2022 | Last updated: | Apr 2024 |
| Keywords: | [GFP reporter] [E. coli] [riboswitches] | Manuscripts: | |||
| Organizations: |
Northwestern University, Evanston, Illinois (United States)
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| Purpose: | Measuring fluorescence of yxjA riboswitch-SFGFP reporter construct in E. coli. Samples were grown in media with or without 2-aminopurine ligand to observe changes in fluorescence in response to riboswitch regulation. | ||||
| Conclusion: | The yxjA riboswitch central helix needs to base pair to perform strand exchange and control gene expression in response to ligand binding. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | 3 replicates tested for each construct/condition, bulk fluorescence data collected for each sample using a plate reader, calibrated relative fluorescence units into MFEL units using 8-peak Rainbow particle calibration beads, QC beads run on flow cytometer prior to performing experiment. | ||||
