Experiment Overview
| Repository ID: | FR-FCM-Z4VH | Experiment name: | Proliferative and anti-apoptotic fractions in maturing hematopoietic cell lineages and their role in homeostasis of normal bone marrow | MIFlowCyt score: | 84.00% |
| Primary researcher: | Stefan Mestrum | PI/manager: | Mathie Leers | Uploaded by: | Stefan Mestrum |
| Experiment dates: | 2020-06-01 - 2021-01-01 | Dataset uploaded: | Feb 2022 | Last updated: | Feb 2022 |
| Keywords: | [proliferation] [Ki-67] [multiparameter flow cytometry] [normal bone marrow] [Bcl-2] [anti-apoptosis] [maturation] [hematopoietic cell lineages] | Manuscripts: | [35429122] [35429122] [PMC9540078] |
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| Organizations: |
GROW-School for Oncology and Developmental Biology, Maastricht University Medical Center, Department of Molecular Cell Biology, Maastricht, (Nederland)
Zuyderland Medical Center, Department of Clinical Chemistry & Hematology, Sittard-Geleen, (Nederland) |
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| Purpose: | Recent developments in clinical flow cytometry allow the simultaneous assessment of proliferative and anti-apoptotic activity in the different hematopoietic cell lineages and during their maturation process. This can further advance the flow cytometric diagnosis of myeloid malignancies. In this study we established indicative reference values for the Ki-67 proliferation index and Bcl-2 anti-apoptotic index in blast cells, as well as maturing erythroid, myeloid and monocytic cells from normal bone marrow (BM). Furthermore, the cell fractions co-expressing both proliferation and anti-apoptotic markers were quantified. | ||||
| Conclusion: | We present a novel application of an earlier developed assay that allows the simultaneous determination of the Ki-67 proliferative and Bcl-2 anti-apoptotic indices in maturing hematopoietic cell populations of the BM. Their differential expression levels during the maturation process were in accordance with the demand and lifespan of these cell populations. The indicative reference values established in this study can act as a baseline for further cell biological and biomedical studies involving hematological malignancies. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | To correct for background staining by the conjugated primary antibodies, a negative control using FITC-directly conjugated to non-relevant, isotype immunoglobulins was used. To prevent staining abnormalities caused by ageing of the sample, flow cytometry was performed within 24 hours of receival of the femoral heads. To ensure sufficient cells for flow cytometric analyses, a white blood cell count of approximately 20 x 109 cells/L (quantified with Sysmex XN-9000) was used preferentially. The instrument setup was performed according to standard procedures. Verification of the optical alignment and fluidics system of the Navios™ Flow Cytometer was performed using Flow-Check™ Pro Fluorospheres (Beckman Coulter). The compensation for each fluorochrome was established using Flow-Set™ Pro Fluorospheres (Beckman Coulter) and was performed weekly. | ||||
Experiment variables
| Individuals | |
|---|---|
| · Femoral Heads | Botkop 10 150620 stm 00011561 001.LMD · Botkop 10 150620 stm 00011562 002.LMD · Botkop 10 150620 stm 00011563 003.LMD · Botkop 10 150620 stm 00011564 004.LMD · Botkop 10 150620 stm 00011565 005.LMD · Botkop 10 150620 stm 00011566 006.LMD · Botkop 10 150620 stm 00011567 007.LMD · Botkop 2 250220 stm 00011112 001.LMD · Botkop 2 250220 stm 00011113 002.LMD · Botkop 2 250220 stm 00011114 003.LMD · Botkop 2 250220 stm 00011115 004.LMD · Botkop 2 250220 stm 00011116 005.LMD · Botkop 2 250220 stm 00011117 006.LMD · Botkop 2 250220 stm 00011118 007.LMD · Botkop 4 100320 stm 00011200 001.LMD · Botkop 4 100320 stm 00011201 002.LMD · Botkop 4 100320 stm 00011202 003.LMD · Botkop 4 100320 stm 00011203 004.LMD · Botkop 4 100320 stm 00011204 005.LMD · Botkop 4 100320 stm 00011205 006.LMD · Botkop 4 100320 stm 00011206 007.LMD · Botkop 5 090620 stm 00011508 001.LMD · Botkop 5 090620 stm 00011509 002.LMD · Botkop 5 090620 stm 00011510 003.LMD · Botkop 5 090620 stm 00011511 004.LMD · Botkop 5 090620 stm 00011512 005.LMD · Botkop 5 090620 stm 00011513 006.LMD · Botkop 5 090620 stm 00011514 007.LMD · Botkop 7 100620 stm 00011518 001.LMD · Botkop 7 100620 stm 00011519 002.LMD · Botkop 7 100620 stm 00011520 003.LMD · Botkop 7 100620 stm 00011521 004.LMD · Botkop 7 100620 stm 00011522 005.LMD · Botkop 7 100620 stm 00011523 006.LMD · Botkop 7 100620 stm 00011524 007.LMD · Botkop 8 100620 stm 00011525 001.LMD · Botkop 8 100620 stm 00011526 002.LMD · Botkop 8 100620 stm 00011527 003.LMD · Botkop 8 100620 stm 00011528 004.LMD · Botkop 8 100620 stm 00011529 005.LMD · Botkop 8 100620 stm 00011530 006.LMD · Botkop 8 100620 stm 00011531 007.LMD · Botkop 9 120620 stm 00011542 001.LMD · Botkop 9 120620 stm 00011543 002.LMD · Botkop 9 120620 stm 00011544 003.LMD · Botkop 9 120620 stm 00011545 004.LMD · Botkop 9 120620 stm 00011546 005.LMD · Botkop 9 120620 stm 00011547 006.LMD · Botkop 9 120620 stm 00011548 007.LMD · botkop 1 030320 rby 00011163 001.LMD · botkop 1 030320 rby 00011164 002.LMD · botkop 1 030320 rby 00011165 003.LMD · botkop 1 030320 rby 00011166 004.LMD · botkop 1 030320 rby 00011167 005.LMD · botkop 1 030320 rby 00011168 006.LMD · botkop 1 030320 rby 00011169 007.LMD · botkop 3 060320 rby 00011181 001.LMD · botkop 3 060320 rby 00011182 002.LMD · botkop 3 060320 rby 00011183 003.LMD · botkop 3 060320 rby 00011184 004.LMD · botkop 3 060320 rby 00011185 005.LMD · botkop 3 060320 rby 00011186 006.LMD · botkop 3 060320 rby 00011187 007.LMD · botkop 6 rby 00011006 001.LMD · botkop 6 rby 00011007 002.LMD · botkop 6 rby 00011008 003.LMD · botkop 6 rby 00011009 004.LMD · botkop 6 rby 00011010 005.LMD · botkop 6 rby 00011011 006.LMD · botkop 6 rby 00011012 007.LMD · Botkop 11 150620 stm 00011570 001.LMD · Botkop 11 150620 stm 00011571 002.LMD · Botkop 11 150620 stm 00011572 003.LMD · Botkop 11 150620 stm 00011573 004.LMD · Botkop 11 150620 stm 00011574 005.LMD · Botkop 11 150620 stm 00011575 006.LMD · Botkop 11 150620 stm 00011576 007.LMD · Botkop 13 180620 stm 00011640 001.LMD · Botkop 13 180620 stm 00011641 002.LMD · Botkop 13 180620 stm 00011642 003.LMD · Botkop 13 180620 stm 00011643 004.LMD · Botkop 13 180620 stm 00011644 005.LMD · Botkop 13 180620 stm 00011645 006.LMD · Botkop 13 180620 stm 00011646 007.LMD · Botkop 14 190620 001.LMD · Botkop 14 190620 002.LMD · Botkop 14 190620 003.LMD · Botkop 14 190620 004.LMD · Botkop 14 190620 005.LMD · Botkop 14 190620 006.LMD · Botkop 14 190620 007.LMD · Botkop 17 220620 rby 00011691 001.LMD · Botkop 17 220620 rby 00011692 002.LMD · Botkop 17 220620 rby 00011693 003.LMD · Botkop 17 220620 rby 00011694 004.LMD · Botkop 17 220620 rby 00011695 005.LMD · Botkop 17 220620 rby 00011696 006.LMD · Botkop 17 220620 rby 00011697 007.LMD · Botkop 20 250620 rby 00011737 001.LMD · Botkop 20 250620 rby 00011738 002.LMD · Botkop 20 250620 rby 00011739 003.LMD · Botkop 20 250620 rby 00011740 004.LMD · Botkop 20 250620 rby 00011741 005.LMD · Botkop 20 250620 rby 00011742 006.LMD · Botkop 20 250620 rby 00011743 007.LMD · Botkop 21 290620 stm 00011777 001.LMD · Botkop 21 290620 stm 00011778 002.LMD · Botkop 21 290620 stm 00011779 003.LMD · Botkop 21 290620 stm 00011780 004.LMD · Botkop 21 290620 stm 00011781 005.LMD · Botkop 21 290620 stm 00011782 006.LMD · Botkop 21 290620 stm 00011783 007.LMD · Botkop 22 290620 rby 00011784 001.LMD · Botkop 22 290620 rby 00011785 002.LMD · Botkop 22 290620 rby 00011786 003.LMD · Botkop 22 290620 rby 00011787 004.LMD · Botkop 22 290620 rby 00011788 005.LMD · Botkop 22 290620 rby 00011789 006.LMD · Botkop 22 290620 rby 00011790 007.LMD · Botkop 23 290620 rby 00011791 001.LMD · Botkop 23 290620 rby 00011792 002.LMD · Botkop 23 290620 rby 00011793 003.LMD · Botkop 23 290620 rby 00011794 004.LMD · Botkop 23 290620 rby 00011795 005.LMD · Botkop 23 290620 rby 00011796 006.LMD · Botkop 23 290620 rby 00011797 007.LMD · Botkop 31 stm 00012065 001.LMD · Botkop 31 stm 00012066 002.LMD · Botkop 31 stm 00012067 003.LMD · Botkop 31 stm 00012068 004.LMD · Botkop 31 stm 00012069 005.LMD · Botkop 31 stm 00012070 006.LMD · Botkop 31 stm 00012071 007.LMD · Botkop 32 stm 00012081 001.LMD · Botkop 32 stm 00012082 002.LMD · Botkop 32 stm 00012083 003.LMD · Botkop 32 stm 00012084 004.LMD · Botkop 32 stm 00012085 005.LMD · Botkop 32 stm 00012086 006.LMD · Botkop 32 stm 00012087 007.LMD · Botkop 34 rby 00012096 002.LMD · Botkop 34 rby 00012097 003.LMD · Botkop 34 rby 00012098 004.LMD · Botkop 34 rby 00012099 005.LMD · Botkop 34 rby 00012100 006.LMD · Botkop 34 rby 00012101 007.LMD · Botkop 35 stm 00012102 001.LMD · Botkop 35 stm 00012103 002.LMD · Botkop 35 stm 00012104 003.LMD · Botkop 35 stm 00012105 004.LMD · Botkop 35 stm 00012106 005.LMD · Botkop 35 stm 00012107 006.LMD · Botkop 35 stm 00012108 007.LMD · Botkop 36 stm 00012114 001.LMD · Botkop 36 stm 00012115 002.LMD · Botkop 36 stm 00012116 003.LMD · Botkop 36 stm 00012117 004.LMD · Botkop 36 stm 00012118 005.LMD · Botkop 36 stm 00012119 006.LMD · Botkop 36 stm 00012120 007.LMD · Botkop 40 stm 00012151 001.LMD · Botkop 40 stm 00012152 002.LMD · Botkop 40 stm 00012153 003.LMD · Botkop 40 stm 00012154 004.LMD · Botkop 40 stm 00012155 005.LMD · Botkop 40 stm 00012156 006.LMD · Botkop 40 stm 00012157 007.LMD · Botkop 41 stm 00012161 001.LMD · Botkop 41 stm 00012162 002.LMD · Botkop 41 stm 00012163 003.LMD · Botkop 41 stm 00012164 004.LMD · Botkop 41 stm 00012165 005.LMD · Botkop 41 stm 00012166 006.LMD · Botkop 41 stm 00012167 007.LMD · Botkop 42 stm 00012168 001.LMD · Botkop 42 stm 00012169 002.LMD · Botkop 42 stm 00012170 003.LMD · Botkop 42 stm 00012171 004.LMD · Botkop 42 stm 00012172 005.LMD · Botkop 42 stm 00012173 006.LMD · Botkop 42 stm 00012174 007.LMD · Botkop 43 stm 00012212 001.LMD · Botkop 43 stm 00012213 002.LMD · Botkop 43 stm 00012214 003.LMD · Botkop 43 stm 00012215 004.LMD · Botkop 43 stm 00012216 005.LMD · Botkop 43 stm 00012217 006.LMD · Botkop 43 stm 00012218 007.LMD · Botkop 44 stm 00012225 001.LMD · Botkop 44 stm 00012226 002.LMD · Botkop 44 stm 00012227 003.LMD · Botkop 44 stm 00012228 004.LMD · Botkop 44 stm 00012229 005.LMD · Botkop 44 stm 00012230 006.LMD · Botkop 44 stm 00012231 007.LMD · Botkop 45 stm 00012233 001.LMD · Botkop 45 stm 00012234 002.LMD · Botkop 45 stm 00012235 003.LMD · Botkop 45 stm 00012236 004.LMD · Botkop 45 stm 00012237 005.LMD · Botkop 45 stm 00012238 006.LMD · Botkop 45 stm 00012239 007.LMD · Botkop 46 stm 00012243 001.LMD · Botkop 46 stm 00012244 002.LMD · Botkop 46 stm 00012245 003.LMD · Botkop 46 stm 00012246 004.LMD · Botkop 46 stm 00012247 005.LMD · Botkop 46 stm 00012248 006.LMD · Botkop 46 stm 00012249 007.LMD · Botkop 47 stm 00012253 001.LMD · Botkop 47 stm 00012254 002.LMD · Botkop 47 stm 00012255 003.LMD · Botkop 47 stm 00012256 004.LMD · Botkop 47 stm 00012257 005.LMD · Botkop 47 stm 00012258 006.LMD · Botkop 47 stm 00012259 007.LMD · Botkop 49 stm 00012297 001.LMD · Botkop 49 stm 00012298 002.LMD · Botkop 49 stm 00012299 003.LMD · Botkop 49 stm 00012300 004.LMD · Botkop 49 stm 00012301 005.LMD · Botkop 49 stm 00012302 006.LMD · Botkop 49 stm 00012303 007.LMD · Botkop 50 stm 00012309 001.LMD · Botkop 50 stm 00012310 002.LMD · Botkop 50 stm 00012311 003.LMD · Botkop 50 stm 00012312 004.LMD · Botkop 50 stm 00012313 005.LMD · Botkop 50 stm 00012314 006.LMD · Botkop 50 stm 00012315 007.LMD · botkop 12 18-06-20 rby 00011633 001.LMD · botkop 12 18-06-20 rby 00011634 002.LMD · botkop 12 18-06-20 rby 00011635 003.LMD · botkop 12 18-06-20 rby 00011636 004.LMD · botkop 12 18-06-20 rby 00011637 005.LMD · botkop 12 18-06-20 rby 00011638 006.LMD · botkop 12 18-06-20 rby 00011639 007.LMD · botkop 15 190620 rby 00011671 001.LMD · botkop 15 190620 rby 00011672 002.LMD · botkop 15 190620 rby 00011673 003.LMD · botkop 15 190620 rby 00011674 004.LMD · botkop 15 190620 rby 00011675 005.LMD · botkop 15 190620 rby 00011676 006.LMD · botkop 15 190620 rby 00011677 007.LMD · botkop 16 220620 rby 00011684 001.LMD · botkop 16 220620 rby 00011685 002.LMD · botkop 16 220620 rby 00011686 003.LMD · botkop 16 220620 rby 00011687 004.LMD · botkop 16 220620 rby 00011688 005.LMD · botkop 16 220620 rby 00011689 006.LMD · botkop 16 220620 rby 00011690 007.LMD · botkop 18 230620 rby 00011702 001.LMD · botkop 18 230620 rby 00011703 002.LMD · botkop 18 230620 rby 00011704 003.LMD · botkop 18 230620 rby 00011705 004.LMD · botkop 18 230620 rby 00011706 005.LMD · botkop 18 230620 rby 00011707 006.LMD · botkop 18 230620 rby 00011708 007.LMD · botkop 19 230620 rby 00011713 001.LMD · botkop 19 230620 rby 00011714 002.LMD · botkop 19 230620 rby 00011715 003.LMD · botkop 19 230620 rby 00011716 004.LMD · botkop 19 230620 rby 00011717 005.LMD · botkop 19 230620 rby 00011718 006.LMD · botkop 19 230620 rby 00011719 007.LMD · botkop 24 300620 rby 00011810 001.LMD · botkop 24 300620 rby 00011811 002.LMD · botkop 24 300620 rby 00011812 003.LMD · botkop 24 300620 rby 00011813 004.LMD · botkop 24 300620 rby 00011814 005.LMD · botkop 24 300620 rby 00011815 006.LMD · botkop 24 300620 rby 00011816 007.LMD · botkop 25 300620 rby 00011822 001.LMD · botkop 25 300620 rby 00011823 002.LMD · botkop 25 300620 rby 00011824 003.LMD · botkop 25 300620 rby 00011825 004.LMD · botkop 25 300620 rby 00011826 005.LMD · botkop 25 300620 rby 00011827 006.LMD · botkop 25 300620 rby 00011828 007.LMD · botkop 26 020720 rby 00011837 001.LMD · botkop 26 020720 rby 00011838 002.LMD · botkop 26 020720 rby 00011839 003.LMD · botkop 26 020720 rby 00011840 004.LMD · botkop 26 020720 rby 00011841 005.LMD · botkop 26 020720 rby 00011842 006.LMD · botkop 26 020720 rby 00011843 007.LMD · botkop 27 030720 rby 00011845 001.LMD · botkop 27 030720 rby 00011846 002.LMD · botkop 27 030720 rby 00011847 003.LMD · botkop 27 030720 rby 00011848 004.LMD · botkop 27 030720 rby 00011849 005.LMD · botkop 27 030720 rby 00011850 006.LMD · botkop 27 030720 rby 00011851 007.LMD · botkop 28 070720 rby 00011869 001.LMD · botkop 28 070720 rby 00011870 002.LMD · botkop 28 070720 rby 00011871 003.LMD · botkop 28 070720 rby 00011872 004.LMD · botkop 28 070720 rby 00011873 005.LMD · botkop 28 070720 rby 00011874 006.LMD · botkop 28 070720 rby 00011875 007.LMD · botkop 29 100720 rby 00011891 001.LMD · botkop 29 100720 rby 00011892 002.LMD · botkop 29 100720 rby 00011893 003.LMD · botkop 29 100720 rby 00011894 004.LMD · botkop 29 100720 rby 00011895 005.LMD · botkop 29 100720 rby 00011896 006.LMD · botkop 29 100720 rby 00011897 007.LMD · botkop 30 100720 rby 00011906 001.LMD · botkop 30 100720 rby 00011907 002.LMD · botkop 30 100720 rby 00011908 003.LMD · botkop 30 100720 rby 00011909 004.LMD · botkop 30 100720 rby 00011910 005.LMD · botkop 30 100720 rby 00011911 006.LMD · botkop 30 100720 rby 00011912 007.LMD · botkop 33 rby 00012088 001.LMD · botkop 33 rby 00012089 002.LMD · botkop 33 rby 00012090 003.LMD · botkop 33 rby 00012091 004.LMD · botkop 33 rby 00012092 005.LMD · botkop 33 rby 00012093 006.LMD · botkop 33 rby 00012094 007.LMD · botkop 34 rby 00012095 001.LMD · botkop 37 240820 rby 00012123 001.LMD · botkop 37 240820 rby 00012124 002.LMD · botkop 37 240820 rby 00012125 003.LMD · botkop 37 240820 rby 00012126 004.LMD · botkop 37 240820 rby 00012127 005.LMD · botkop 37 240820 rby 00012128 006.LMD · botkop 37 240820 rby 00012129 007.LMD · botkop 38 240820 rby 00012132 001.LMD · botkop 38 240820 rby 00012133 002.LMD · botkop 38 240820 rby 00012134 003.LMD · botkop 38 240820 rby 00012135 004.LMD · botkop 38 240820 rby 00012136 005.LMD · botkop 38 240820 rby 00012137 006.LMD · botkop 38 240820 rby 00012138 007.LMD · botkop 39 240820 rby 00012139 001.LMD · botkop 39 240820 rby 00012140 002.LMD · botkop 39 240820 rby 00012141 003.LMD · botkop 39 240820 rby 00012142 004.LMD · botkop 39 240820 rby 00012143 005.LMD · botkop 39 240820 rby 00012144 006.LMD · botkop 39 240820 rby 00012145 007.LMD |
| Timepoints | |
|---|---|
| · After Hip surgery | Botkop 10 150620 stm 00011561 001.LMD · Botkop 10 150620 stm 00011562 002.LMD · Botkop 10 150620 stm 00011563 003.LMD · Botkop 10 150620 stm 00011564 004.LMD · Botkop 10 150620 stm 00011565 005.LMD · Botkop 10 150620 stm 00011566 006.LMD · Botkop 10 150620 stm 00011567 007.LMD · Botkop 2 250220 stm 00011112 001.LMD · Botkop 2 250220 stm 00011113 002.LMD · Botkop 2 250220 stm 00011114 003.LMD · Botkop 2 250220 stm 00011115 004.LMD · Botkop 2 250220 stm 00011116 005.LMD · Botkop 2 250220 stm 00011117 006.LMD · Botkop 2 250220 stm 00011118 007.LMD · Botkop 4 100320 stm 00011200 001.LMD · Botkop 4 100320 stm 00011201 002.LMD · Botkop 4 100320 stm 00011202 003.LMD · Botkop 4 100320 stm 00011203 004.LMD · Botkop 4 100320 stm 00011204 005.LMD · Botkop 4 100320 stm 00011205 006.LMD · Botkop 4 100320 stm 00011206 007.LMD · Botkop 5 090620 stm 00011508 001.LMD · Botkop 5 090620 stm 00011509 002.LMD · Botkop 5 090620 stm 00011510 003.LMD · Botkop 5 090620 stm 00011511 004.LMD · Botkop 5 090620 stm 00011512 005.LMD · Botkop 5 090620 stm 00011513 006.LMD · Botkop 5 090620 stm 00011514 007.LMD · Botkop 7 100620 stm 00011518 001.LMD · Botkop 7 100620 stm 00011519 002.LMD · Botkop 7 100620 stm 00011520 003.LMD · Botkop 7 100620 stm 00011521 004.LMD · Botkop 7 100620 stm 00011522 005.LMD · Botkop 7 100620 stm 00011523 006.LMD · Botkop 7 100620 stm 00011524 007.LMD · Botkop 8 100620 stm 00011525 001.LMD · Botkop 8 100620 stm 00011526 002.LMD · Botkop 8 100620 stm 00011527 003.LMD · Botkop 8 100620 stm 00011528 004.LMD · Botkop 8 100620 stm 00011529 005.LMD · Botkop 8 100620 stm 00011530 006.LMD · Botkop 8 100620 stm 00011531 007.LMD · Botkop 9 120620 stm 00011542 001.LMD · Botkop 9 120620 stm 00011543 002.LMD · Botkop 9 120620 stm 00011544 003.LMD · Botkop 9 120620 stm 00011545 004.LMD · Botkop 9 120620 stm 00011546 005.LMD · Botkop 9 120620 stm 00011547 006.LMD · Botkop 9 120620 stm 00011548 007.LMD · botkop 1 030320 rby 00011163 001.LMD · botkop 1 030320 rby 00011164 002.LMD · botkop 1 030320 rby 00011165 003.LMD · botkop 1 030320 rby 00011166 004.LMD · botkop 1 030320 rby 00011167 005.LMD · botkop 1 030320 rby 00011168 006.LMD · botkop 1 030320 rby 00011169 007.LMD · botkop 3 060320 rby 00011181 001.LMD · botkop 3 060320 rby 00011182 002.LMD · botkop 3 060320 rby 00011183 003.LMD · botkop 3 060320 rby 00011184 004.LMD · botkop 3 060320 rby 00011185 005.LMD · botkop 3 060320 rby 00011186 006.LMD · botkop 3 060320 rby 00011187 007.LMD · botkop 6 rby 00011006 001.LMD · botkop 6 rby 00011007 002.LMD · botkop 6 rby 00011008 003.LMD · botkop 6 rby 00011009 004.LMD · botkop 6 rby 00011010 005.LMD · botkop 6 rby 00011011 006.LMD · botkop 6 rby 00011012 007.LMD · Botkop 11 150620 stm 00011570 001.LMD · Botkop 11 150620 stm 00011571 002.LMD · Botkop 11 150620 stm 00011572 003.LMD · Botkop 11 150620 stm 00011573 004.LMD · Botkop 11 150620 stm 00011574 005.LMD · Botkop 11 150620 stm 00011575 006.LMD · Botkop 11 150620 stm 00011576 007.LMD · Botkop 13 180620 stm 00011640 001.LMD · Botkop 13 180620 stm 00011641 002.LMD · Botkop 13 180620 stm 00011642 003.LMD · Botkop 13 180620 stm 00011643 004.LMD · Botkop 13 180620 stm 00011644 005.LMD · Botkop 13 180620 stm 00011645 006.LMD · Botkop 13 180620 stm 00011646 007.LMD · Botkop 14 190620 001.LMD · Botkop 14 190620 002.LMD · Botkop 14 190620 003.LMD · Botkop 14 190620 004.LMD · Botkop 14 190620 005.LMD · Botkop 14 190620 006.LMD · Botkop 14 190620 007.LMD · Botkop 17 220620 rby 00011691 001.LMD · Botkop 17 220620 rby 00011692 002.LMD · Botkop 17 220620 rby 00011693 003.LMD · Botkop 17 220620 rby 00011694 004.LMD · Botkop 17 220620 rby 00011695 005.LMD · Botkop 17 220620 rby 00011696 006.LMD · Botkop 17 220620 rby 00011697 007.LMD · Botkop 20 250620 rby 00011737 001.LMD · Botkop 20 250620 rby 00011738 002.LMD · Botkop 20 250620 rby 00011739 003.LMD · Botkop 20 250620 rby 00011740 004.LMD · Botkop 20 250620 rby 00011741 005.LMD · Botkop 20 250620 rby 00011742 006.LMD · Botkop 20 250620 rby 00011743 007.LMD · Botkop 21 290620 stm 00011777 001.LMD · Botkop 21 290620 stm 00011778 002.LMD · Botkop 21 290620 stm 00011779 003.LMD · Botkop 21 290620 stm 00011780 004.LMD · Botkop 21 290620 stm 00011781 005.LMD · Botkop 21 290620 stm 00011782 006.LMD · Botkop 21 290620 stm 00011783 007.LMD · Botkop 22 290620 rby 00011784 001.LMD · Botkop 22 290620 rby 00011785 002.LMD · Botkop 22 290620 rby 00011786 003.LMD · Botkop 22 290620 rby 00011787 004.LMD · Botkop 22 290620 rby 00011788 005.LMD · Botkop 22 290620 rby 00011789 006.LMD · Botkop 22 290620 rby 00011790 007.LMD · Botkop 23 290620 rby 00011791 001.LMD · Botkop 23 290620 rby 00011792 002.LMD · Botkop 23 290620 rby 00011793 003.LMD · Botkop 23 290620 rby 00011794 004.LMD · Botkop 23 290620 rby 00011795 005.LMD · Botkop 23 290620 rby 00011796 006.LMD · Botkop 23 290620 rby 00011797 007.LMD · Botkop 31 stm 00012065 001.LMD · Botkop 31 stm 00012066 002.LMD · Botkop 31 stm 00012067 003.LMD · Botkop 31 stm 00012068 004.LMD · Botkop 31 stm 00012069 005.LMD · Botkop 31 stm 00012070 006.LMD · Botkop 31 stm 00012071 007.LMD · Botkop 32 stm 00012081 001.LMD · Botkop 32 stm 00012082 002.LMD · Botkop 32 stm 00012083 003.LMD · Botkop 32 stm 00012084 004.LMD · Botkop 32 stm 00012085 005.LMD · Botkop 32 stm 00012086 006.LMD · Botkop 32 stm 00012087 007.LMD · Botkop 34 rby 00012096 002.LMD · Botkop 34 rby 00012097 003.LMD · Botkop 34 rby 00012098 004.LMD · Botkop 34 rby 00012099 005.LMD · Botkop 34 rby 00012100 006.LMD · Botkop 34 rby 00012101 007.LMD · Botkop 35 stm 00012102 001.LMD · Botkop 35 stm 00012103 002.LMD · Botkop 35 stm 00012104 003.LMD · Botkop 35 stm 00012105 004.LMD · Botkop 35 stm 00012106 005.LMD · Botkop 35 stm 00012107 006.LMD · Botkop 35 stm 00012108 007.LMD · Botkop 36 stm 00012114 001.LMD · Botkop 36 stm 00012115 002.LMD · Botkop 36 stm 00012116 003.LMD · Botkop 36 stm 00012117 004.LMD · Botkop 36 stm 00012118 005.LMD · Botkop 36 stm 00012119 006.LMD · Botkop 36 stm 00012120 007.LMD · Botkop 40 stm 00012151 001.LMD · Botkop 40 stm 00012152 002.LMD · Botkop 40 stm 00012153 003.LMD · Botkop 40 stm 00012154 004.LMD · Botkop 40 stm 00012155 005.LMD · Botkop 40 stm 00012156 006.LMD · Botkop 40 stm 00012157 007.LMD · Botkop 41 stm 00012161 001.LMD · Botkop 41 stm 00012162 002.LMD · Botkop 41 stm 00012163 003.LMD · Botkop 41 stm 00012164 004.LMD · Botkop 41 stm 00012165 005.LMD · Botkop 41 stm 00012166 006.LMD · Botkop 41 stm 00012167 007.LMD · Botkop 42 stm 00012168 001.LMD · Botkop 42 stm 00012169 002.LMD · Botkop 42 stm 00012170 003.LMD · Botkop 42 stm 00012171 004.LMD · Botkop 42 stm 00012172 005.LMD · Botkop 42 stm 00012173 006.LMD · Botkop 42 stm 00012174 007.LMD · Botkop 43 stm 00012212 001.LMD · Botkop 43 stm 00012213 002.LMD · Botkop 43 stm 00012214 003.LMD · Botkop 43 stm 00012215 004.LMD · Botkop 43 stm 00012216 005.LMD · Botkop 43 stm 00012217 006.LMD · Botkop 43 stm 00012218 007.LMD · Botkop 44 stm 00012225 001.LMD · Botkop 44 stm 00012226 002.LMD · Botkop 44 stm 00012227 003.LMD · Botkop 44 stm 00012228 004.LMD · Botkop 44 stm 00012229 005.LMD · Botkop 44 stm 00012230 006.LMD · Botkop 44 stm 00012231 007.LMD · Botkop 45 stm 00012233 001.LMD · Botkop 45 stm 00012234 002.LMD · Botkop 45 stm 00012235 003.LMD · Botkop 45 stm 00012236 004.LMD · Botkop 45 stm 00012237 005.LMD · Botkop 45 stm 00012238 006.LMD · Botkop 45 stm 00012239 007.LMD · Botkop 46 stm 00012243 001.LMD · Botkop 46 stm 00012244 002.LMD · Botkop 46 stm 00012245 003.LMD · Botkop 46 stm 00012246 004.LMD · Botkop 46 stm 00012247 005.LMD · Botkop 46 stm 00012248 006.LMD · Botkop 46 stm 00012249 007.LMD · Botkop 47 stm 00012253 001.LMD · Botkop 47 stm 00012254 002.LMD · Botkop 47 stm 00012255 003.LMD · Botkop 47 stm 00012256 004.LMD · Botkop 47 stm 00012257 005.LMD · Botkop 47 stm 00012258 006.LMD · Botkop 47 stm 00012259 007.LMD · Botkop 49 stm 00012297 001.LMD · Botkop 49 stm 00012298 002.LMD · Botkop 49 stm 00012299 003.LMD · Botkop 49 stm 00012300 004.LMD · Botkop 49 stm 00012301 005.LMD · Botkop 49 stm 00012302 006.LMD · Botkop 49 stm 00012303 007.LMD · Botkop 50 stm 00012309 001.LMD · Botkop 50 stm 00012310 002.LMD · Botkop 50 stm 00012311 003.LMD · Botkop 50 stm 00012312 004.LMD · Botkop 50 stm 00012313 005.LMD · Botkop 50 stm 00012314 006.LMD · Botkop 50 stm 00012315 007.LMD · botkop 12 18-06-20 rby 00011633 001.LMD · botkop 12 18-06-20 rby 00011634 002.LMD · botkop 12 18-06-20 rby 00011635 003.LMD · botkop 12 18-06-20 rby 00011636 004.LMD · botkop 12 18-06-20 rby 00011637 005.LMD · botkop 12 18-06-20 rby 00011638 006.LMD · botkop 12 18-06-20 rby 00011639 007.LMD · botkop 15 190620 rby 00011671 001.LMD · botkop 15 190620 rby 00011672 002.LMD · botkop 15 190620 rby 00011673 003.LMD · botkop 15 190620 rby 00011674 004.LMD · botkop 15 190620 rby 00011675 005.LMD · botkop 15 190620 rby 00011676 006.LMD · botkop 15 190620 rby 00011677 007.LMD · botkop 16 220620 rby 00011684 001.LMD · botkop 16 220620 rby 00011685 002.LMD · botkop 16 220620 rby 00011686 003.LMD · botkop 16 220620 rby 00011687 004.LMD · botkop 16 220620 rby 00011688 005.LMD · botkop 16 220620 rby 00011689 006.LMD · botkop 16 220620 rby 00011690 007.LMD · botkop 18 230620 rby 00011702 001.LMD · botkop 18 230620 rby 00011703 002.LMD · botkop 18 230620 rby 00011704 003.LMD · botkop 18 230620 rby 00011705 004.LMD · botkop 18 230620 rby 00011706 005.LMD · botkop 18 230620 rby 00011707 006.LMD · botkop 18 230620 rby 00011708 007.LMD · botkop 19 230620 rby 00011713 001.LMD · botkop 19 230620 rby 00011714 002.LMD · botkop 19 230620 rby 00011715 003.LMD · botkop 19 230620 rby 00011716 004.LMD · botkop 19 230620 rby 00011717 005.LMD · botkop 19 230620 rby 00011718 006.LMD · botkop 19 230620 rby 00011719 007.LMD · botkop 24 300620 rby 00011810 001.LMD · botkop 24 300620 rby 00011811 002.LMD · botkop 24 300620 rby 00011812 003.LMD · botkop 24 300620 rby 00011813 004.LMD · botkop 24 300620 rby 00011814 005.LMD · botkop 24 300620 rby 00011815 006.LMD · botkop 24 300620 rby 00011816 007.LMD · botkop 25 300620 rby 00011822 001.LMD · botkop 25 300620 rby 00011823 002.LMD · botkop 25 300620 rby 00011824 003.LMD · botkop 25 300620 rby 00011825 004.LMD · botkop 25 300620 rby 00011826 005.LMD · botkop 25 300620 rby 00011827 006.LMD · botkop 25 300620 rby 00011828 007.LMD · botkop 26 020720 rby 00011837 001.LMD · botkop 26 020720 rby 00011838 002.LMD · botkop 26 020720 rby 00011839 003.LMD · botkop 26 020720 rby 00011840 004.LMD · botkop 26 020720 rby 00011841 005.LMD · botkop 26 020720 rby 00011842 006.LMD · botkop 26 020720 rby 00011843 007.LMD · botkop 27 030720 rby 00011845 001.LMD · botkop 27 030720 rby 00011846 002.LMD · botkop 27 030720 rby 00011847 003.LMD · botkop 27 030720 rby 00011848 004.LMD · botkop 27 030720 rby 00011849 005.LMD · botkop 27 030720 rby 00011850 006.LMD · botkop 27 030720 rby 00011851 007.LMD · botkop 28 070720 rby 00011869 001.LMD · botkop 28 070720 rby 00011870 002.LMD · botkop 28 070720 rby 00011871 003.LMD · botkop 28 070720 rby 00011872 004.LMD · botkop 28 070720 rby 00011873 005.LMD · botkop 28 070720 rby 00011874 006.LMD · botkop 28 070720 rby 00011875 007.LMD · botkop 29 100720 rby 00011891 001.LMD · botkop 29 100720 rby 00011892 002.LMD · botkop 29 100720 rby 00011893 003.LMD · botkop 29 100720 rby 00011894 004.LMD · botkop 29 100720 rby 00011895 005.LMD · botkop 29 100720 rby 00011896 006.LMD · botkop 29 100720 rby 00011897 007.LMD · botkop 30 100720 rby 00011906 001.LMD · botkop 30 100720 rby 00011907 002.LMD · botkop 30 100720 rby 00011908 003.LMD · botkop 30 100720 rby 00011909 004.LMD · botkop 30 100720 rby 00011910 005.LMD · botkop 30 100720 rby 00011911 006.LMD · botkop 30 100720 rby 00011912 007.LMD · botkop 33 rby 00012088 001.LMD · botkop 33 rby 00012089 002.LMD · botkop 33 rby 00012090 003.LMD · botkop 33 rby 00012091 004.LMD · botkop 33 rby 00012092 005.LMD · botkop 33 rby 00012093 006.LMD · botkop 33 rby 00012094 007.LMD · botkop 34 rby 00012095 001.LMD · botkop 37 240820 rby 00012123 001.LMD · botkop 37 240820 rby 00012124 002.LMD · botkop 37 240820 rby 00012125 003.LMD · botkop 37 240820 rby 00012126 004.LMD · botkop 37 240820 rby 00012127 005.LMD · botkop 37 240820 rby 00012128 006.LMD · botkop 37 240820 rby 00012129 007.LMD · botkop 38 240820 rby 00012132 001.LMD · botkop 38 240820 rby 00012133 002.LMD · botkop 38 240820 rby 00012134 003.LMD · botkop 38 240820 rby 00012135 004.LMD · botkop 38 240820 rby 00012136 005.LMD · botkop 38 240820 rby 00012137 006.LMD · botkop 38 240820 rby 00012138 007.LMD · botkop 39 240820 rby 00012139 001.LMD · botkop 39 240820 rby 00012140 002.LMD · botkop 39 240820 rby 00012141 003.LMD · botkop 39 240820 rby 00012142 004.LMD · botkop 39 240820 rby 00012143 005.LMD · botkop 39 240820 rby 00012144 006.LMD · botkop 39 240820 rby 00012145 007.LMD |
