Experiment Overview
| Repository ID: | FR-FCM-Z4WC | Experiment name: | Characterization of iPSC-derived macrophages (surface marker profile, Figure 5) | MIFlowCyt score: | 32.86% |
| Primary researcher: | Mania Ackermann | PI/manager: | Mania Ackermann | Uploaded by: | Mania Ackermann |
| Experiment dates: | 2020-09-09 - | Dataset uploaded: | Sep 2021 | Last updated: | Sep 2021 |
| Keywords: | [pluripotent stem cells] [iPSC-derived macrophages] [cell-therapy] | Manuscripts: | |||
| Organizations: |
Hannover Medical School, Institute of Experimental Hematology, Hannover, (Germany)
|
||||
| Purpose: | Characterization of surface markers expressed on iPSC-derived macrophages produced in stirred tank bioreactors (method described in Ackermann et al., Nature Communcations, 2018) | ||||
| Conclusion: | Macrophages produced from hCD34iPSC in stirred tank bioreactors show a highly pure surface maker profile of CD45+/CD11b+/CD14+/CD163+/TRA-1-60-. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Calibration of baseline settings, PMT-Voltages and quality control of the LSR II cytometer was regularly performed using the Becton-Dickinson CS&T beads and FACSDiva software according to the manufacturer. | ||||
