Experiment Overview
| Repository ID: | FR-FCM-Z4WE | Experiment name: | Characterization of iPSC-derived macrophages (phagocytosis, Figure 6) | MIFlowCyt score: | 38.17% |
| Primary researcher: | Mania Ackermann | PI/manager: | Mania Ackermann | Uploaded by: | Mania Ackermann |
| Experiment dates: | 2020-09-02 - 2020-09-09 | Dataset uploaded: | Sep 2021 | Last updated: | Sep 2021 |
| Keywords: | [phagocytosis] [human induced pluripotent stem cells] [iPSC-derived macrophages] | Manuscripts: | |||
| Organizations: |
Hannover Medical School, Institute of Experimental Hematology, Hannover, (Germany)
|
||||
| Purpose: | Characterization of the phagocytotic activity of macrophages derived from human iPSC (hCD34iPSC) by using pHrodo Red E. coli BioParticles (TM, ThermoFisher) | ||||
| Conclusion: | Macrophages produced from hCD34iPSC in stirred tank bioreactors actively phagocytose pHrodo™ Red E. coli BioParticles™. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Calibration of baseline settings, PMT-Voltages and quality control of the LSR II cytometer was regularly performed using the Becton-Dickinson CS&T beads and FACSDiva software according to the manufacturer. | ||||
