Experiment Overview
| Repository ID: | FR-FCM-Z4X4 | Experiment name: | CD4 Fluorochrome stain index in Aurora 5L - Immunotrol vs Fresh human Blood | MIFlowCyt score: | 49.25% |
| Primary researcher: | Alexis Perez Gonzalez | PI/manager: | Alexis Perez Gonzalez | Uploaded by: | Alexis Perez Gonzalez |
| Experiment dates: | 2021-08-23 - 2021-08-23 | Dataset uploaded: | Nov 2021 | Last updated: | Nov 2021 |
| Keywords: | [Cellular autofluorescence] [induced autofluorescence] [Fluorescence resolution] [Fluorochrome Brightness] | Manuscripts: | |||
| Organizations: |
University of Melbourne, Microbiology and Immunology, Melbourne, (Australia)
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| Purpose: | Fluorochrome brightness via CD4 stain indexes in Aurora 5L. Brightness comparison of Beckman Coulter Immuno-TROL and fresh human blood. | ||||
| Conclusion: | Stain Index (and fluorochrome brightness) is inversely proportional to carrier autofluorescence levels. | ||||
| Comments: | All samples were acquired in Cytek Aurora 5L (UV16-V16-B14-YG10-R8) at Cytek recommended assay settings. All FCS are raw files acquired after successful instrument embedded QC. Immuno-Trol samples are labelled as "Imt", fresh human blood samples are labelled as "huB". Samples were stained with 26 unique fluorochromes conjugated to CD4 antibody (clone SK3; BD Horizon evaluation kit cat #566352, Becton Dickinson, Franklin Lakes, NJ, USA). | ||||
| Funding: | NA | ||||
| Quality control: | Experiments done after successful QC in Cytek Aurora 5L | ||||
