Experiment Overview
| Repository ID: | FR-FCM-Z54Q | Experiment name: | variability of isogenic lymphoblastoid cell line | MIFlowCyt score: | 31.50% |
| Primary researcher: | Zsuzsanna Hevessy | PI/manager: | Zsuzsanna Hevessy | Uploaded by: | Zsuzsanna Hevessy |
| Experiment dates: | 2015-12-10 - 2016-12-20 | Dataset uploaded: | Mar 2022 | Last updated: | Mar 2022 |
| Keywords: | [variability] [LCL] [isogenic] [trio] | Manuscripts: | [30894562] [PMC6426863] | ||
| Organizations: |
University of Debrecen, Faculty of Medicine, Department of Laboratory Medicine, Debrecen, (Hungary)
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| Purpose: | The purpose of the experiments was to better understand the variability of lymphoblastoid cells on genetic, epigenetic, proteomic, and flow cytometric levels. In these experiments, three LCL cell lines derived from a trio (mother, father, and child) together with five isogenic LCL cell lines derived from a single individual were investigated by flow cytometry. Measurement details and results are presented in the published article. | ||||
| Conclusion: | Based on the performed experiments even isogenic cell lines derived from a single individual has some degree of variability on the investigated parameters. This variability needs to be taken into accont if different LCL cell lines are investigated. | ||||
| Comments: | None | ||||
| Funding: | None | ||||
| Quality control: | FACS Canto II was calibrated daily, using Cytometer Setup and Tracking fluorescent microbeads (Cat No. 641319, Becton Dickinson, San Jose, CA) and Autocomp software as recommended by the manufacturer. Antibodies were titrated to obtain the optimal staining index and to reduce spillover. | ||||
