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Comments:
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Saccharomyces cerevisiae, a species of yeast, is an important model organism and a typical fungal representative for studies on eukaryotes. Flow cytometry is an essential and efficient approach for the analysis of the cell cycle in yeast, and refining the cell cycle resolution is important. However, the resolution refinement is possibly subject to multiple factors, including altered enzymatic treatment and medium variation, which had been seldomly investigated. In this study, flow cytometry combined with multiple enzymatic treatments and various media are performed to determine how this resolution is affected. We show that the resolution is significantly altered under multiple enzymatic conditions. In addition, various media also results in the fluctuation in the resolution. Therefore, different enzymes are confirmed to refine the resolution with varied efficiencies, which is suspicious of due to the alternated culture media with different amino nitrogen concentrations, impacting the protein content in the yeast cell wall, and leading to the changed permeability of cell wall and the variable resolution. This study provides beneficial technical information about the influences of the adjustments of media and enzymes on refining the cell cycle resolution of yeast and, probably, on other fungi, which should be included in the consideration in future research.
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