Repository ID:
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FR-FCM-Z59C
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Experiment name:
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Integration of scRNA-seq with CyTOF data revealing activated CAR-NK cells
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MIFlowCyt score:
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54.08%
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Primary researcher:
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Katy Rezvani |
PI/manager:
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Katy Rezvani |
Uploaded by:
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Katy Rezvani |
Experiment dates:
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2018-04-30 - 2022-04-21 |
Dataset uploaded:
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Apr 2022
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Last updated:
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Apr 2022
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Keywords:
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[CyTOF; mass cytometry; flow cytometry; standardization]
[NK cells; CAR NK; integration]
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Manuscripts:
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Organizations:
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MD Anderson Cancer Center, Houston, Texas (United States)
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Purpose:
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Chimeric antigen receptor (CAR)-transduced natural killer (NK) cells have demonstrated promising efficacy and safety in killing cells in CD19-positive lymphoid tumors. To understand why certain NK cells are more effective than others, we compared the molecular profiles of three groups of NK cells: 1) wildtype non-transduced (NT-NK), 2) transduced with CD19CAR, and 3) transduced with interleukin-15 (IL15). |
Conclusion:
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Integrating scRNA-seq and CyTOF data using bindSC led to the discovery of a subset of highly activated CD19CAR NK cells. This finding may help quantify the therapeutic value of a CAR-NK cell project and reveal mechanisms that can be further leveraged to improve the efficacy of the treatment.
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Comments:
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N.A
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Funding:
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N.A
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Quality control:
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Integration of scRNA-seq with CyTOF data revealing activated CAR-NK cells |
N.A, everting is performed using default pipeline |