Experiment Overview
| Repository ID: | FR-FCM-Z59C | Experiment name: | Integration of scRNA-seq with CyTOF data revealing activated CAR-NK cells | MIFlowCyt score: | 54.08% |
| Primary researcher: | Katy Rezvani | PI/manager: | Katy Rezvani | Uploaded by: | Katy Rezvani |
| Experiment dates: | 2018-04-30 - 2022-04-21 | Dataset uploaded: | Apr 2022 | Last updated: | Apr 2022 |
| Keywords: | [CyTOF; mass cytometry; flow cytometry; standardization] [NK cells; CAR NK; integration] | Manuscripts: | |||
| Organizations: |
MD Anderson Cancer Center, Houston, Texas (United States)
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| Purpose: | Chimeric antigen receptor (CAR)-transduced natural killer (NK) cells have demonstrated promising efficacy and safety in killing cells in CD19-positive lymphoid tumors. To understand why certain NK cells are more effective than others, we compared the molecular profiles of three groups of NK cells: 1) wildtype non-transduced (NT-NK), 2) transduced with CD19CAR, and 3) transduced with interleukin-15 (IL15). | ||||
| Conclusion: | Integrating scRNA-seq and CyTOF data using bindSC led to the discovery of a subset of highly activated CD19CAR NK cells. This finding may help quantify the therapeutic value of a CAR-NK cell project and reveal mechanisms that can be further leveraged to improve the efficacy of the treatment. | ||||
| Comments: | N.A | ||||
| Funding: | N.A | ||||
| Quality control: | Integration of scRNA-seq with CyTOF data revealing activated CAR-NK cells | N.A, everting is performed using default pipeline | |||
