Experiment Overview
| Repository ID: | FR-FCM-Z5BC | Experiment name: | Number of events for ploidy level analysis and genome size estimation | MIFlowCyt score: | 81.75% |
| Primary researcher: | Petr Koutecky | PI/manager: | Petr Koutecky | Uploaded by: | Petr Koutecky |
| Experiment dates: | 2019-04-30 - 2022-02-16 | Dataset uploaded: | Apr 2022 | Last updated: | Aug 2022 |
| Keywords: | [flow cytometry] [DAPI] [Plants] [Nuclei count] | Manuscripts: | |||
| Organizations: |
University of South Bohemia, Faculty of Science, Ceske Budejovice, (Czechia)
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| Purpose: | Determining of minimum number of events (total and per each peak) needed for precise estimation of ploidy level and/or genome size in plant samples. | ||||
| Conclusion: | Total count of 2,000 events and/or 600 events per peak is required for samples with low to moderate level of debris and two peaks. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | All samples were collected from the same individuals and analyzed on the same instrument. Summary of basic statistics (peak index or peak mean, coefficients of variation, number of evets per peak) is provided. The histograms were analyzed using histogram modeling approach without any setting defined by the user. | ||||
Experiment variables
| Individuals | |
|---|---|
| · Sol1 | Bellis+Solanum200_1.fcs · Bellis+Solanum200_6.fcs · SolanumPseudocaps01.fcs · SolanumPseudocaps06.fcs |
| · Sol2 | Bellis+Solanum200_2.fcs · Bellis+Solanum200_7.fcs · SolanumPseudocaps02.fcs · SolanumPseudocaps07.fcs |
| · Sol3 | Bellis+Solanum200_3.fcs · Bellis+Solanum200_8.fcs · SolanumPseudocaps03.fcs · SolanumPseudocaps08.fcs |
| · Sol4 | Bellis+Solanum200_4.fcs · Bellis+Solanum200_9.fcs · SolanumPseudocaps04.fcs · SolanumPseudocaps09.fcs |
| · Sol5 | Bellis+Solanum200_10.fcs · Bellis+Solanum200_5.fcs · SolanumPseudocaps05.fcs · SolanumPseudocaps10.fcs |
| · Sorb1 | SorbusInterm+B300_1.fcs · SorbusInterm+B300_6.fcs |
| · Sorb2 | SorbusInterm+B300_2.fcs · SorbusInterm+B300_7.fcs |
| · Sorb3 | SorbusInterm+B300_3.fcs · SorbusInterm+B300_8.fcs |
| · Sorb4 | SorbusInterm+B300_4.fcs · SorbusInterm+B300_9.fcs |
| · Sorb5 | SorbusInterm+B300_10.fcs · SorbusInterm+B300_5.fcs |
