Experiment Overview
| Repository ID: | FR-FCM-Z5E3 | Experiment name: | NMO rituximab study | MIFlowCyt score: | 19.50% |
| Primary researcher: | Jakob Theorell | PI/manager: | Jakob Theorell | Uploaded by: | Jakob Theorell |
| Experiment dates: | 2018-06-14 - 2020-01-21 | Dataset uploaded: | May 2022 | Last updated: | May 2022 |
| Keywords: | None | Manuscripts: | |||
| Organizations: | None | ||||
| Purpose: | This repository is associated to the publication Rituximab abrogates aquaporin-4-specific germinal centre activity in patients with Neuromyelitis optica spectrum disorders by Valentina Damato et al (PNAS 2022). It is a collection of lymphocytes from paired fine-needle aspirate and blood samples from humans with Neuromyelitis optics or controls as well as compensation control files. The current article focuses on B-cells, and thus the panels are skewed in a B-cell direction. Importantly, the associated analysis (FlowJo) files, files for annotation of the samples and also the resulting sheets with information that is used as input for the figures can all be found at: https://github.com/jtheorell/Damato_NMO_RTX. | ||||
| Conclusion: | None | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | The panels were generated with FMOs which were subsequently used in the establishment of the compensation, which varied from donor pair to donor pair. The pairs are internally consistent as they were always acquired at the same time, but the different pairs have been ac queried with sometimes different panels, and in all instances slightly different settings due to changes in machinery over the two-year study period. For this reason are all compensation matrices individual for each sample pair and the gates are also individually adjusted in many cases. The associated flowjo files are present at https://github.com/jtheorell/Damato_NMO_RTX . | ||||
