Experiment Overview
| Repository ID: | FR-FCM-Z5E8 | Experiment name: | BM-1 (2018-4) Bone Marrow Microphysiological System Independent Measures Power Calculation | MIFlowCyt score: | 82.17% |
| Primary researcher: | Emilyanne Leonard | PI/manager: | Emilyanne Leonard | Uploaded by: | Emilyanne Leonard |
| Experiment dates: | 2018-07-04 - 2018-08-16 | Dataset uploaded: | May 2022 | Last updated: | Jun 2022 |
| Keywords: | [human bone marrow] [bone marrow on a chip] [microphysiological system] | Manuscripts: | |||
| Organizations: |
AstraZeneca, Cambridge, Cambridgeshire (United Kingdom)
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| Purpose: | The purpose was to test the power calculation of n=6 in independent measures of cells from the BM MPS system using compound AZ1. This tested if an n=6: gives less variable data and increases confidence in responses seen, allows detection of toxicity at lower doses of compound AZ1 than previously tested, and allows confident detection of recovery. | ||||
| Conclusion: | The power calculation of n=6 is appropriate for this independent measures design. However, the next experiment should investigate a repeated measures design to reduce variability chip to chip. | ||||
| Comments: | In this independent measures study design, the cells were sampled from the scaffold well of the chip, meaning the chip was terminally harvested and the next timepoint's samples were from different chips. These samples are marked with an 'S'. Cells were also samples from the media well of the chip, labelled as 'F' for floating. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | To standardize voltage settings across timepoints, single color controls were included each day to calculate compensation. Each dosed group had 6 replicates. | ||||
Experiment variables
| Timepoints | |
|---|---|
| · D0 | Test Samples_23F_003.fcs · Test Samples_23S_004.fcs · Test Samples_24F_005.fcs · Test Samples_24S_006.fcs · Test Samples_27F_007.fcs · Test Samples_27S_008.fcs · Test Samples_28F_009.fcs · Test Samples_28S_010.fcs · Test Samples_38F_011.fcs · Test Samples_38S_012.fcs · Test Samples_7F_001.fcs · Test Samples_7S_002.fcs |
| · D14 | FMO_CD13 BV421 FMO_005.fcs · FMO_CD16 APC FMO_008.fcs · FMO_CD235a BV786 FMO_002.fcs · FMO_CD34 APC-Cy7 FMO_009.fcs · FMO_CD36 BV605 FMO_001.fcs · FMO_CD38 BUV395 FMO_006.fcs · FMO_CD41A PE-Cy7 FMO_003.fcs · FMO_CD71 PE FMO_007.fcs · FMO_Unstained FMO_010.fcs · FMO_eFluor506 Viability FMO_004.fcs · Test Samples_10F_019.fcs · Test Samples_10S_020.fcs · Test Samples_13F_021.fcs · Test Samples_13S_022.fcs · Test Samples_14F_023.fcs · Test Samples_14S_024.fcs · Test Samples_19F_025.fcs · Test Samples_19S_026.fcs · Test Samples_1F_011.fcs · Test Samples_1S_012.fcs · Test Samples_20F_027.fcs · Test Samples_20S_028.fcs · Test Samples_29F_029.fcs · Test Samples_29S_030.fcs · Test Samples_30F_031.fcs · Test Samples_30S_032.fcs · Test Samples_33F_033.fcs · Test Samples_33S_034.fcs · Test Samples_34F_035.fcs · Test Samples_34S_036.fcs · Test Samples_39F_037.fcs · Test Samples_39S_038.fcs · Test Samples_3F_013.fcs · Test Samples_3S_014.fcs · Test Samples_40F_039.fcs · Test Samples_40S_040.fcs · Test Samples_43F_041.fcs · Test Samples_43S_042.fcs · Test Samples_44F_043.fcs · Test Samples_44S_044.fcs · Test Samples_48F_045.fcs · Test Samples_48S_046.fcs · Test Samples_4F_015.fcs · Test Samples_4S_016.fcs · Test Samples_9F_017.fcs · Test Samples_9S_018.fcs |
| · D28 | FMO_CD13 BV421 FMO_005.fcs-(1) · FMO_CD16 APC FMO_008.fcs-(1) · FMO_CD235a BV786 FMO_002.fcs-(1) · FMO_CD34 APC-Cy7 FMO_009.fcs-(1) · FMO_CD36 BV605 FMO_001.fcs-(1) · FMO_CD38 BUV395 FMO_006.fcs-(1) · FMO_CD41A PE-Cy7 FMO_003.fcs-(1) · FMO_CD71 PE FMO_007.fcs-(1) · FMO_Unstained FMO_010.fcs-(1) · FMO_eFluor506 Viability FMO_004.fcs-(1) · Test Samples_11F_017.fcs · Test Samples_11S_018.fcs · Test Samples_12F_019.fcs · Test Samples_12S_020.fcs · Test Samples_17F_021.fcs · Test Samples_17S_022.fcs · Test Samples_18F_023.fcs · Test Samples_18S_024.fcs · Test Samples_21F_025.fcs · Test Samples_21S_026.fcs · Test Samples_22F_027.fcs · Test Samples_22S_028.fcs · Test Samples_25F_029.fcs · Test Samples_25S_030.fcs · Test Samples_26F_031.fcs · Test Samples_26S_032.fcs · Test Samples_2F_011.fcs · Test Samples_2S_012.fcs · Test Samples_31F_033.fcs · Test Samples_31S_034.fcs · Test Samples_32F_035.fcs · Test Samples_32S_036.fcs · Test Samples_35F_037.fcs · Test Samples_35S_038.fcs · Test Samples_41F_039.fcs · Test Samples_41S_040.fcs · Test Samples_42F_041.fcs · Test Samples_42S_042.fcs · Test Samples_45F_043.fcs · Test Samples_45S_044.fcs · Test Samples_46F_045.fcs · Test Samples_46S_046.fcs · Test Samples_5F_013.fcs · Test Samples_5S_014.fcs · Test Samples_6F_015.fcs · Test Samples_6S_016.fcs |
