Experiment Overview
| Repository ID: | FR-FCM-Z5ET | Experiment name: | BM-2 (2018-5) Bone Marrow Microphysiological System within Chip Monitoring Test (Repeated Measures) | MIFlowCyt score: | 92.59% |
| Primary researcher: | Emilyanne Leonard | PI/manager: | Emilyanne Leonard | Uploaded by: | Emilyanne Leonard |
| Experiment dates: | 2018-07-30 - 2018-09-11 | Dataset uploaded: | May 2022 | Last updated: | Jun 2022 |
| Keywords: | [bone marrow on a chip] [microphysiological system] [human bone] | Manuscripts: | [37063297] [PMC10103791] | ||
| Organizations: |
AstraZeneca, Cambridge, Cambridgeshire (United Kingdom)
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| Purpose: | The purpose was to test within chip monitoring of cells within the BM MPS using extended AZ1 treatment and recovery. This tested if monitoring one chip through the duration of the study was possible and reduced variability, called repeated measures. This experiment also tested if repeated measures allow detection of toxicity at doses lower than 10uM AZ1 and allow confident detection of recovery. | ||||
| Conclusion: | The repeated measures study design minimizes chip variability, and treatment effects can be detected by measuring cells from the media alone. | ||||
| Comments: | In this repeated measures study design, the cells were sampled from the media well of the chip at each timepoint. In addition, the scaffold well was also sampled on the last timepoint at Day 28, where the samples are marked with an 'S'. Cells were also samples from the media well of the chip on Day 28, labelled as 'F' for floating. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | To standardize voltage settings across timepoints, single color controls were included each day to calculate compensation. Each dosed group had 6 replicates. | ||||
Experiment variables
| Timepoints | |
|---|---|
| · D0 | FMO_CD13 BV421 FMO_005.fcs · FMO_CD16 APC FMO_008.fcs · FMO_CD235a BV786 FMO_002.fcs · FMO_CD34 APC-Cy7 FMO_009.fcs · FMO_CD36 BV605 FMO_001.fcs · FMO_CD38 BUV395 FMO_006.fcs · FMO_CD41A PE-Cy7 FMO_003.fcs · FMO_CD71 PE FMO_007.fcs · FMO_Unstained FMO_010.fcs · FMO_eFluor506 Viability FMO_004.fcs · Test Samples_10_014.fcs · Test Samples_13_015.fcs · Test Samples_14_016.fcs · Test Samples_19_017.fcs · Test Samples_20_018.fcs · Test Samples_23_019.fcs · Test Samples_29_020.fcs · Test Samples_30_021.fcs · Test Samples_33_022.fcs · Test Samples_34_023.fcs · Test Samples_37 Check_024.fcs · Test Samples_39L_030.fcs · Test Samples_3_011.fcs · Test Samples_40L_031.fcs · Test Samples_44L_032.fcs · Test Samples_47L_033.fcs · Test Samples_4_012.fcs · Test Samples_9_013.fcs |
| · D7 | FMO_CD13 BV421 FMO_005.fcs-(1) · FMO_CD16 APC FMO_008.fcs-(1) · FMO_CD235a BV786 FMO_002.fcs-(1) · FMO_CD34 APC-Cy7 FMO_009.fcs-(1) · FMO_CD36 BV605 FMO_001.fcs-(1) · FMO_CD38 BUV395 FMO_006.fcs-(1) · FMO_CD41A PE-Cy7 FMO_003.fcs-(1) · FMO_CD71 PE FMO_007.fcs-(1) · FMO_Unstained FMO_010.fcs-(1) · FMO_eFluor506 Viability FMO_004.fcs-(1) · Test Samples_10_014.fcs-(1) · Test Samples_13_015.fcs-(1) · Test Samples_14_016.fcs-(1) · Test Samples_19_017.fcs-(1) · Test Samples_20_018.fcs-(1) · Test Samples_23_019.fcs-(1) · Test Samples_29_020.fcs-(1) · Test Samples_30_021.fcs-(1) · Test Samples_33_022.fcs-(1) · Test Samples_34_023.fcs-(1) · Test Samples_37_024.fcs · Test Samples_39_025.fcs · Test Samples_3_011.fcs-(1) · Test Samples_40_026.fcs · Test Samples_44_027.fcs · Test Samples_47_028.fcs · Test Samples_4_012.fcs-(1) · Test Samples_9_013.fcs-(1) |
| · D14 | FMO_CD13 BV421 FMO_005.fcs-(2) · FMO_CD16 APC FMO_008.fcs-(2) · FMO_CD235a BV786 FMO_002.fcs-(2) · FMO_CD34 APC-Cy7 FMO_009.fcs-(2) · FMO_CD36 BV605 FMO_001.fcs-(2) · FMO_CD38 BUV395 FMO_006.fcs-(2) · FMO_CD41A PE-Cy7 FMO_003.fcs-(2) · FMO_CD71 PE FMO_007.fcs-(2) · FMO_Unstained FMO_010.fcs-(2) · FMO_eFluor506 Viability FMO_004.fcs-(2) · Test Samples_10_014.fcs-(2) · Test Samples_13_015.fcs-(2) · Test Samples_14_016.fcs-(2) · Test Samples_19_017.fcs-(2) · Test Samples_20_018.fcs-(2) · Test Samples_23_019.fcs-(2) · Test Samples_29_020.fcs-(2) · Test Samples_30_021.fcs-(2) · Test Samples_33_022.fcs-(2) · Test Samples_34_023.fcs-(2) · Test Samples_37_024.fcs-(1) · Test Samples_39_025.fcs-(1) · Test Samples_3_011.fcs-(2) · Test Samples_40_026.fcs-(1) · Test Samples_44_027.fcs-(1) · Test Samples_47_028.fcs-(1) · Test Samples_4_012.fcs-(2) · Test Samples_9_013.fcs-(2) |
| · D21 | FMO_CD13 BV421 FMO_005.fcs-(3) · FMO_CD16 APC FMO_008.fcs-(3) · FMO_CD235a BV786 FMO_002.fcs-(3) · FMO_CD34 APC-Cy7 FMO_009.fcs-(3) · FMO_CD36 BV605 FMO_001.fcs-(3) · FMO_CD38 BUV395 FMO_006.fcs-(3) · FMO_CD41A PE-Cy7 FMO_003.fcs-(3) · FMO_CD71 PE FMO_007.fcs-(3) · FMO_Unstained FMO_010.fcs-(3) · FMO_eFluor506 Viability FMO_004.fcs-(3) · Test Samples_10_014.fcs-(3) · Test Samples_13_015.fcs-(3) · Test Samples_14_016.fcs-(3) · Test Samples_19_017.fcs-(3) · Test Samples_20_018.fcs-(3) · Test Samples_23_019.fcs-(3) · Test Samples_29_020.fcs-(3) · Test Samples_30_021.fcs-(3) · Test Samples_33_022.fcs-(3) · Test Samples_34_023.fcs-(3) · Test Samples_37_024.fcs-(2) · Test Samples_39_025.fcs-(2) · Test Samples_3_011.fcs-(3) · Test Samples_40_026.fcs-(2) · Test Samples_44_027.fcs-(2) · Test Samples_47_028.fcs-(2) · Test Samples_4_012.fcs-(3) · Test Samples_9_013.fcs-(3) |
| · D28 | FMO_CD13 BV421 FMO_005.fcs-(4) · FMO_CD16 APC FMO_008.fcs-(4) · FMO_CD235a BV786 FMO_002.fcs-(4) · FMO_CD34 APC-Cy7 FMO_009.fcs-(4) · FMO_CD36 BV605 FMO_001.fcs-(4) · FMO_CD38 BUV395 FMO_006.fcs-(4) · FMO_CD41A PE-Cy7 FMO_003.fcs-(4) · FMO_CD71 PE FMO_007.fcs-(4) · FMO_Unstained FMO_010.fcs-(4) · FMO_eFluor506 Viability FMO_004.fcs-(4) · Test Samples_10F_017.fcs · Test Samples_10S_018.fcs · Test Samples_13F_019.fcs · Test Samples_13S_020.fcs · Test Samples_14F_021.fcs · Test Samples_14S_022.fcs · Test Samples_19F_023.fcs · Test Samples_19S_024.fcs · Test Samples_20F_025.fcs · Test Samples_20S_026.fcs · Test Samples_23F_027.fcs · Test Samples_23S_028.fcs · Test Samples_29F_029.fcs · Test Samples_29S_030.fcs · Test Samples_30F_031.fcs · Test Samples_30S_032.fcs · Test Samples_33F_033.fcs · Test Samples_33S_034.fcs · Test Samples_34F_035.fcs · Test Samples_34S_036.fcs · Test Samples_37F_037.fcs · Test Samples_37S_038.fcs · Test Samples_39F_039.fcs · Test Samples_39S_040.fcs · Test Samples_3F_011.fcs · Test Samples_3S_012.fcs · Test Samples_40F_041.fcs · Test Samples_40S_042.fcs · Test Samples_44F_043.fcs · Test Samples_44S_044.fcs · Test Samples_47F_045.fcs · Test Samples_47S_046.fcs · Test Samples_4F_013.fcs · Test Samples_4S_014.fcs · Test Samples_9F_015.fcs · Test Samples_9S_016.fcs |
| Doses | |
|---|---|
| · Control | FMO_CD13 BV421 FMO_005.fcs · FMO_CD16 APC FMO_008.fcs · FMO_CD235a BV786 FMO_002.fcs · FMO_CD34 APC-Cy7 FMO_009.fcs · FMO_CD36 BV605 FMO_001.fcs · FMO_CD38 BUV395 FMO_006.fcs · FMO_CD41A PE-Cy7 FMO_003.fcs · FMO_CD71 PE FMO_007.fcs · FMO_Unstained FMO_010.fcs · FMO_eFluor506 Viability FMO_004.fcs · Test Samples_13_015.fcs · Test Samples_20_018.fcs · Test Samples_29_020.fcs · Test Samples_34_023.fcs · Test Samples_40L_031.fcs · Test Samples_9_013.fcs · FMO_CD13 BV421 FMO_005.fcs-(1) · FMO_CD16 APC FMO_008.fcs-(1) · FMO_CD235a BV786 FMO_002.fcs-(1) · FMO_CD34 APC-Cy7 FMO_009.fcs-(1) · FMO_CD36 BV605 FMO_001.fcs-(1) · FMO_CD38 BUV395 FMO_006.fcs-(1) · FMO_CD41A PE-Cy7 FMO_003.fcs-(1) · FMO_CD71 PE FMO_007.fcs-(1) · FMO_Unstained FMO_010.fcs-(1) · FMO_eFluor506 Viability FMO_004.fcs-(1) · Test Samples_13_015.fcs-(1) · Test Samples_20_018.fcs-(1) · Test Samples_29_020.fcs-(1) · Test Samples_34_023.fcs-(1) · Test Samples_40_026.fcs · Test Samples_9_013.fcs-(1) · FMO_CD13 BV421 FMO_005.fcs-(2) · FMO_CD16 APC FMO_008.fcs-(2) · FMO_CD235a BV786 FMO_002.fcs-(2) · FMO_CD34 APC-Cy7 FMO_009.fcs-(2) · FMO_CD36 BV605 FMO_001.fcs-(2) · FMO_CD38 BUV395 FMO_006.fcs-(2) · FMO_CD41A PE-Cy7 FMO_003.fcs-(2) · FMO_CD71 PE FMO_007.fcs-(2) · FMO_Unstained FMO_010.fcs-(2) · FMO_eFluor506 Viability FMO_004.fcs-(2) · Test Samples_13_015.fcs-(2) · Test Samples_20_018.fcs-(2) · Test Samples_29_020.fcs-(2) · Test Samples_34_023.fcs-(2) · Test Samples_40_026.fcs-(1) · Test Samples_9_013.fcs-(2) · FMO_CD13 BV421 FMO_005.fcs-(3) · FMO_CD16 APC FMO_008.fcs-(3) · FMO_CD235a BV786 FMO_002.fcs-(3) · FMO_CD34 APC-Cy7 FMO_009.fcs-(3) · FMO_CD36 BV605 FMO_001.fcs-(3) · FMO_CD38 BUV395 FMO_006.fcs-(3) · FMO_CD41A PE-Cy7 FMO_003.fcs-(3) · FMO_CD71 PE FMO_007.fcs-(3) · FMO_Unstained FMO_010.fcs-(3) · FMO_eFluor506 Viability FMO_004.fcs-(3) · Test Samples_13_015.fcs-(3) · Test Samples_20_018.fcs-(3) · Test Samples_29_020.fcs-(3) · Test Samples_34_023.fcs-(3) · Test Samples_40_026.fcs-(2) · Test Samples_9_013.fcs-(3) · FMO_CD13 BV421 FMO_005.fcs-(4) · FMO_CD16 APC FMO_008.fcs-(4) · FMO_CD235a BV786 FMO_002.fcs-(4) · FMO_CD34 APC-Cy7 FMO_009.fcs-(4) · FMO_CD36 BV605 FMO_001.fcs-(4) · FMO_CD38 BUV395 FMO_006.fcs-(4) · FMO_CD41A PE-Cy7 FMO_003.fcs-(4) · FMO_CD71 PE FMO_007.fcs-(4) · FMO_Unstained FMO_010.fcs-(4) · FMO_eFluor506 Viability FMO_004.fcs-(4) · Test Samples_13F_019.fcs · Test Samples_13S_020.fcs · Test Samples_20F_025.fcs · Test Samples_20S_026.fcs · Test Samples_29F_029.fcs · Test Samples_29S_030.fcs · Test Samples_34F_035.fcs · Test Samples_34S_036.fcs · Test Samples_40F_041.fcs · Test Samples_40S_042.fcs · Test Samples_9F_015.fcs · Test Samples_9S_016.fcs |
| · AZ1 1uM | Test Samples_14_016.fcs · Test Samples_23_019.fcs · Test Samples_30_021.fcs · Test Samples_39L_030.fcs · Test Samples_44L_032.fcs · Test Samples_4_012.fcs · Test Samples_14_016.fcs-(1) · Test Samples_23_019.fcs-(1) · Test Samples_30_021.fcs-(1) · Test Samples_39_025.fcs · Test Samples_44_027.fcs · Test Samples_4_012.fcs-(1) · Test Samples_14_016.fcs-(2) · Test Samples_23_019.fcs-(2) · Test Samples_30_021.fcs-(2) · Test Samples_39_025.fcs-(1) · Test Samples_44_027.fcs-(1) · Test Samples_4_012.fcs-(2) · Test Samples_14_016.fcs-(3) · Test Samples_23_019.fcs-(3) · Test Samples_30_021.fcs-(3) · Test Samples_39_025.fcs-(2) · Test Samples_44_027.fcs-(2) · Test Samples_4_012.fcs-(3) · Test Samples_14F_021.fcs · Test Samples_14S_022.fcs · Test Samples_23F_027.fcs · Test Samples_23S_028.fcs · Test Samples_30F_031.fcs · Test Samples_30S_032.fcs · Test Samples_39F_039.fcs · Test Samples_39S_040.fcs · Test Samples_44F_043.fcs · Test Samples_44S_044.fcs · Test Samples_4F_013.fcs · Test Samples_4S_014.fcs |
| · AZ1 10uM | Test Samples_10_014.fcs · Test Samples_19_017.fcs · Test Samples_33_022.fcs · Test Samples_37 Check_024.fcs · Test Samples_3_011.fcs · Test Samples_47L_033.fcs · Test Samples_10_014.fcs-(1) · Test Samples_19_017.fcs-(1) · Test Samples_33_022.fcs-(1) · Test Samples_37_024.fcs · Test Samples_3_011.fcs-(1) · Test Samples_47_028.fcs · Test Samples_10_014.fcs-(2) · Test Samples_19_017.fcs-(2) · Test Samples_33_022.fcs-(2) · Test Samples_37_024.fcs-(1) · Test Samples_3_011.fcs-(2) · Test Samples_47_028.fcs-(1) · Test Samples_10_014.fcs-(3) · Test Samples_19_017.fcs-(3) · Test Samples_33_022.fcs-(3) · Test Samples_37_024.fcs-(2) · Test Samples_3_011.fcs-(3) · Test Samples_47_028.fcs-(2) · Test Samples_10F_017.fcs · Test Samples_10S_018.fcs · Test Samples_19F_023.fcs · Test Samples_19S_024.fcs · Test Samples_33F_033.fcs · Test Samples_33S_034.fcs · Test Samples_37F_037.fcs · Test Samples_37S_038.fcs · Test Samples_3F_011.fcs · Test Samples_3S_012.fcs · Test Samples_47F_045.fcs · Test Samples_47S_046.fcs |
