Experiment Overview
| Repository ID: | FR-FCM-Z5FF | Experiment name: | bdEV counting from AD and control patients | MIFlowCyt score: | 74.00% |
| Primary researcher: | Yiyao Huang | PI/manager: | Kenneth Witwer | Uploaded by: | Yiyao Huang |
| Experiment dates: | 2020-06-02 - 2020-06-02 | Dataset uploaded: | Jun 2022 | Last updated: | Jun 2022 |
| Keywords: | [proteomics] [exosomes] [Extracellular vesicles] [microvesicles] [brain] [Alzheimer’s disease] [ectosomes] [central nervous system] [cell of origin markers] | Manuscripts: | |||
| Organizations: |
Johns Hopkins University, Medicine, Baltimore, (USA)
|
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| Purpose: | The concentration and size profile of 10K and EV preparations from AD and control patients were measured by the side scatter using NFCM (Flow NanoAnalyzer, NanoFCM, Inc.). | ||||
| Conclusion: | Overall, the concentration of recovered bdEVs was not affected by AD. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | The instrument was pre-calibrated for concentration with 200 nm polystyrene beads. The DPBS used for sample resuspension was used as blank control. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · 10K | A1-10K.fcs · A10-10K.fcs · A11-10K.fcs · A12-10K.fcs · A13-10K.fcs · A14-10K.fcs · A15-10K.fcs · A16-10K.fcs · A17-10K.fcs · A18-10K.fcs · A19-10K.fcs · A2-10K.fcs · A20-10K.fcs · A21-10K.fcs · A22-10K.fcs · A23-10K.fcs · A3-10K.fcs · A4-10K.fcs · A5-10K.fcs · A6-10K.fcs · A7-10K.fcs · A8-10K.fcs · A9-10K.fcs · B1-10K.fcs · B2-10K.fcs · B3-10K.fcs · B4-10K.fcs · B5-10K.fcs · B6-10K.fcs · B7-10K.fcs |
| · EVs | A1-EVs.fcs · A10-EVs-2.fcs · A11-EVs.fcs · A12-EVs.fcs · A13-EVs.fcs · A14-EVs.fcs · A15-EVs.fcs · A16-EVs.fcs · A17-EVs.fcs · A18-EVs.fcs · A19-EVs.fcs · A2-EVs.fcs · A20-EVs.fcs · A21-EVs.fcs · A22-EVs.fcs · A23-EVs.fcs · A3-EVs.fcs · A4-EVs.fcs · A5-EVs.fcs · A6-EVs.fcs · A7-EVs.fcs · A9-EVs.fcs · B1-EVs.fcs · B2-EVs.fcs · B3-EVs.fcs · B4-EVs.fcs · B5-EVs.fcs · B6-EVs.fcs · B7-EVs.fcs |
| · Control | PBS.fcs · beads.fcs |
| Individuals | |
|---|---|
| · A1 | A1-EVs.fcs · A1-10K.fcs |
| · A2 | A2-10K.fcs · A2-EVs.fcs |
| · A3 | A3-10K.fcs · A3-EVs.fcs |
| · A4 | A4-10K.fcs · A4-EVs.fcs |
| · A5 | A5-10K.fcs · A5-EVs.fcs |
| · A6 | A6-10K.fcs · A6-EVs.fcs |
| · A7 | A7-10K.fcs · A7-EVs.fcs |
| · A8 | A8-10K.fcs |
| · A9 | A9-10K.fcs · A9-EVs.fcs |
| · A10 | A10-10K.fcs · A10-EVs-2.fcs |
| · A11 | A11-10K.fcs · A11-EVs.fcs |
| · A12 | A12-10K.fcs · A12-EVs.fcs |
| · A13 | A13-10K.fcs · A13-EVs.fcs |
| · A14 | A14-10K.fcs · A14-EVs.fcs |
| · A15 | A15-10K.fcs · A15-EVs.fcs |
| · A16 | A16-10K.fcs · A16-EVs.fcs |
| · A17 | A17-10K.fcs · A17-EVs.fcs |
| · A18 | A18-10K.fcs · A18-EVs.fcs |
| · A19 | A19-10K.fcs · A19-EVs.fcs |
| · A20 | A20-10K.fcs · A20-EVs.fcs |
| · A21 | A21-10K.fcs · A21-EVs.fcs |
| · A22 | A22-10K.fcs · A22-EVs.fcs |
| · A23 | A23-10K.fcs · A23-EVs.fcs |
| · B1 | B1-10K.fcs · B1-EVs.fcs |
| · B2 | B2-10K.fcs · B2-EVs.fcs |
| · B3 | B3-10K.fcs · B3-EVs.fcs |
| · B4 | B4-10K.fcs · B4-EVs.fcs |
| · B5 | B5-10K.fcs · B5-EVs.fcs |
| · B6 | B6-10K.fcs · B6-EVs.fcs |
| · B7 | B7-10K.fcs · B7-EVs.fcs |
