Experiment Overview
| Repository ID: | FR-FCM-Z5LC | Experiment name: | Characterisation of the immune repertoire of a humanised transgenic mouse through immunophenotyping and high-throughput sequencing (splenocytes) | MIFlowCyt score: | 29.25% |
| Primary researcher: | Spela Binter | PI/manager: | Spela Binter | Uploaded by: | Spela Binter |
| Experiment dates: | 2019-10-09 - 2019-10-17 | Dataset uploaded: | Jul 2022 | Last updated: | Jul 2022 |
| Keywords: | [B-cell] [Immunophenotyping] [transgenic mouse] | Manuscripts: | |||
| Organizations: |
Kymab Ltd., Infectious Disease & Vaccines, Cambridge, (United Kingdom)
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| Purpose: | In this study, we phenotypically characterised B-cell populations from the Intelliselect® Transgenic mouse (Kymouse) demonstrating full B-cell development competence. | ||||
| Conclusion: | Our combined sequence and structural analysis indicates that the naïve Kymouse BCR repertoire is diverse with key similarities to human repertoires, while immunophenotyping confirms that selected naïve B-cells are able to go through complete development. | ||||
| Comments: | n/a | ||||
| Funding: | This work was funded by the Bill & Melinda Gates Foundation, OPP1159947. The funder did not play any role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. | ||||
| Quality control: | n=12 mixed spleen and lymph node samples from 12 mice was used for this study | ||||
