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Notice: Temporary Suspension of New Experiment Creation
We have temporarily disabled the creation of new experiments as we are continuously running out of space. This issue has been impacting both uploads and downloads from FlowRepository. By taking this step, we aim to make downloads of existing data more reliable.
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Experiment Overview

Repository ID: FR-FCM-Z5LE Experiment name: Heterogeneity of the human CD8+ memory T cell pool MIFlowCyt score: 15.00%
Primary researcher: Simone Puccio PI/manager: Simone Puccio Uploaded by: Simone Puccio
Experiment dates: 2022-07-27 - Dataset uploaded: Jul 2022 Last updated: Jul 2022
Keywords: None Manuscripts: [33046887]
Organizations: None
Purpose: We aimed to investigate heterogeneity of the human CD8+ memory T cell pool at the protein level, and to confirm at the protein level the findings we obtained by scRNA-Seq. Thus we designed a high-dimensional flow-cytometry panel based on the cluster signature markers CCR7, LEF1, CD161, GZMB and GZMK. This panel was also equipped to detect memory and effector differentiation markers (CD27, CD28, CD45RO, CD127 and T-bet), activation markers (CD38 and HLA-DR), inhibitory receptors (PD-1 and TIGIT) and markers of tissue residency (CD69 and CD103) in CD8+ T cells isolated from the peripheral blood (PB) and tissues like bone marrow (BM), lymph nodes (LN) and lung.
Conclusion: None
Comments: None
Funding: Not disclosed
Quality control: None
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