Experiment Overview
| Repository ID: | FR-FCM-Z5LV | Experiment name: | Human neutrophil degranulation | MIFlowCyt score: | 53.75% |
| Primary researcher: | Shuang Peng | PI/manager: | Shuang Peng | Uploaded by: | Shuang Peng |
| Experiment dates: | 2017-10-02 - 2022-05-30 | Dataset uploaded: | Aug 2022 | Last updated: | Aug 2022 |
| Keywords: | [human neutrophils] [CD63] [degranulation] [CD66b] | Manuscripts: | |||
| Organizations: |
The University of Bern, Bern, (Switzerland)
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| Purpose: | To determine neutrophil degranulation by cell surface staining of CD63 (surrogate marker for azurophilic granules) and/or CD66b ( surrogate marker for specific granules). | ||||
| Conclusion: | Upon neutrophil activation, cell surface expression of CD63 and CD66b are increased. High expression of RhoH and myosin IIA inhibitors suppress neutrophil degranulation, as reflected by reduced cell surface expression of CD63 and/or CD66b by flow cytometry. | ||||
| Comments: | Isolated neutrophils from healthy donors and cystic fibrosis patients; Neutrophils pre-treated with cell culture medium, healthy serum and CF serum before activation; Neutrophils pre-treated with different myosin IIA inhibitors before activation; | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Isotype controls were used at the same concentration: APC-conjugated Mouse IgG1, κ isotype control (clone MOPC-21; BioLegend), FITC-conjugated Mouse IgM, κ isotype control (clone MM-30; BioLegend) | ||||
Experiment variables
| Conditions | |
|---|---|
| · patients | CF patient GM+C5a.fcs · CF patient Ig complex.fcs · CF patient LPS.fcs · CF patient control.fcs · CF patient fMLP.fcs · CF patient PMA.fcs · Healthy C5a.fcs · Healthy LPS.fcs · Healthy control.fcs · Healthy donor GM+C5a.fcs · Healthy donor GM+LPS.fcs · Healthy donor Ig complex.fcs · Healthy donor control.fcs · Healthy donor fMLP.fcs · PMA.fcs · Patient C5a.fcs · Patient LPS.fcs · Patient PMA.fcs · Patient control.fcs · Tube_001.fcs · Tube_001.fcs-(1) · Tube_002.fcs · Tube_002.fcs-(1) · Tube_003.fcs · Tube_003.fcs-(1) · Tube_004.fcs · Tube_004.fcs-(1) · Tube_005.fcs · Tube_005.fcs-(1) · Tube_006.fcs · Tube_006.fcs-(1) · Tube_007.fcs · Tube_007.fcs-(1) · Tube_008.fcs · Tube_008.fcs-(1) · Tube_009.fcs · Tube_010.fcs · Tube_011.fcs · Tube_012.fcs · CF GM+C5a.fcs · CF PMA.fcs · CF control.fcs · HD C5a.fcs · HD GM+C5a.fcs · HD PMA.fcs · HD PMA.fcs-(1) · HD control.fcs |
| · serum pretreated | CF serum GM+C5a.fcs · CF serum PMA.fcs · CF serum PMA.fcs-(1) · CF serum c5a.fcs · CF serum control .fcs · CF serum unstimulated.fcs · CF serum unstimulation.fcs · HD serum GM+C5a.fcs · HD serum PMA.fcs · HD serum unstimulation.fcs · HD unstimulated.fcs · Medium C5a.fcs · Medium GM+C5a.fcs · Medium PMA.fcs · Medium PMA_001.fcs · Medium control.fcs · RPMI GM+C5a.fcs · RPMI PMA.fcs · RPMI unstimulated.fcs · healthy serum PMA.fcs · healthy serum c5a.fcs · medium PMA.fcs · medium c5a.fcs · medium control.fcs · unstained.fcs |
| Sample Type | |
|---|---|
| · human neutrophils | 4951 GM+C5a.fcs · 4951 PMA.fcs · 4951 isotype GM+C5a.fcs · 4951 isotype PMA.fcs · 4951 isotype untreated.fcs · 4951 untreated.fcs · CF patient GM+C5a.fcs · CF patient Ig complex.fcs · CF patient LPS.fcs · CF patient control.fcs · CF patient fMLP.fcs · CF patient PMA.fcs · Control ab unstimulated.fcs · GM+C5A.fcs · Healthy C5a.fcs · Healthy LPS.fcs · Healthy PMA.fcs · Healthy control.fcs · Healthy donor GM+C5a.fcs · Healthy donor GM+LPS.fcs · Healthy donor Ig complex.fcs · Healthy donor PMA.fcs · Healthy donor control.fcs · Healthy donor fMLP.fcs · Isotype GM + C5a.fcs · Isotype GM+C5a without cytoB.fcs · Isotype GM+C5a.fcs · Isotype GM+C5a.fcs-(1) · Isotype PMA without cytoB.fcs · Isotype PMA.fcs · Isotype PMA.fcs-(1) · Isotype PMA.fcs-(2) · Isotype control without cytoB.fcs · Isotype control.fcs · Isotype control.fcs-(1) · Isotype untreated.fcs · PMA.fcs · Patient C5a.fcs · Patient LPS.fcs · Patient PMA.fcs · Patient control.fcs · Tube_001.fcs · Tube_001.fcs-(1) · Tube_002.fcs · Tube_002.fcs-(1) · Tube_003.fcs · Tube_003.fcs-(1) · Tube_004.fcs · Tube_004.fcs-(1) · Tube_005.fcs · Tube_005.fcs-(1) · Tube_006.fcs · Tube_006.fcs-(1) · Tube_007.fcs · Tube_007.fcs-(1) · Tube_008.fcs · Tube_008.fcs-(1) · Tube_009.fcs · Tube_010.fcs · Tube_011.fcs · Tube_012.fcs · control ab GM+C5a.fcs · control ab PMA.fcs · untreated.fcs · CF GM+C5a.fcs · CF PMA.fcs · CF control.fcs · CF serum GM+C5a.fcs · CF serum PMA.fcs · CF serum PMA.fcs-(1) · CF serum c5a.fcs · CF serum control .fcs · CF serum unstimulated.fcs · CF serum unstimulation.fcs · HD C5a.fcs · HD GM+C5a.fcs · HD PMA.fcs · HD PMA.fcs-(1) · HD control.fcs · HD serum GM+C5a.fcs · HD serum PMA.fcs · HD serum unstimulation.fcs · HD unstimulated.fcs · Medium C5a.fcs · Medium GM+C5a.fcs · Medium PMA.fcs · Medium PMA_001.fcs · Medium control.fcs · RPMI GM+C5a.fcs · RPMI PMA.fcs · RPMI unstimulated.fcs · healthy serum PMA.fcs · healthy serum c5a.fcs · medium PMA.fcs · medium c5a.fcs · medium control.fcs · unstained.fcs |
