Experiment Overview
| Repository ID: | FR-FCM-Z5M3 | Experiment name: | Hoxb8 neutrophil differentiation | MIFlowCyt score: | 53.75% |
| Primary researcher: | Shuang Peng | PI/manager: | Shuang Peng | Uploaded by: | Shuang Peng |
| Experiment dates: | 2017-10-02 - 2022-05-30 | Dataset uploaded: | Aug 2022 | Last updated: | Aug 2022 |
| Keywords: | [differentiation] [neutrophils] [Ly6G] | Manuscripts: | |||
| Organizations: |
The University of Bern, Bern, (Switzerland)
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| Purpose: | To determine the maturation of the differentiated neutrophils by measuring the cell surface expression of Ly6G using flow cytometry. | ||||
| Conclusion: | On day 5, these cells were well differentiated into neutrophils, as shown by the increased expression of Ly6G. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Undifferentiated cells and unstained samples were included. | ||||
Experiment variables
| Conditions | |
|---|---|
| · D0 | EV D0.fcs · EV D0.fcs-(1) · HA-RhoH D0.fcs · HA-RhoH D0.fcs-(1) · HA-RhoH D0.fcs-(2) · HA-RhoH K51A D0.fcs · K51A HA-RhoH D0.fcs · K51A HA-RhoH D0.fcs-(1) · KO D0.fcs · RhoH KO D0.fcs · RhoH KO D0.fcs-(1) · RhoH KO EV D0.fcs · WT D0 .fcs · WT D0.fcs · WT D0.fcs-(1) · shCtrl D0.fcs · shCtrl D0.fcs-(1) · shCtrl D0.fcs-(2) · shMyh9 D0.fcs · shMyh9 D0.fcs-(1) · shMyh9 D0.fcs-(2) |
| · D5 | EV D5.fcs · EV D5.fcs-(1) · HA-RhoH D5.fcs · HA-RhoH D5.fcs-(1) · HA-RhoH D5.fcs-(2) · HA-RhoH K51A D5.fcs · K51A HA-RhoH D5.fcs · K51A HA-RhoH D5.fcs-(1) · KO D5.fcs · RhoH KO D5.fcs · RhoH KO D5.fcs-(1) · RhoH KO EV D5.fcs · WT D5.fcs · WT D5.fcs-(1) · WT D5.fcs-(2) · shCtrl D5.fcs · shCtrl D5.fcs-(1) · shCtrl D5.fcs-(2) · shMyh9 D5.fcs · shMyh9 D5.fcs-(1) · shMyh9 D5.fcs-(2) |
| · unstained | D5 unstain.fcs · HA-RhoH unstain.fcs · K51A HA-RhoH unstain.fcs · RhoH KO EV unstain.fcs · RhoH KO unstained.fcs · WT unstained.fcs · shCtrl unstain.fcs · shMyh9 unstain.fcs |
| Sample Type | |
|---|---|
| · Mouse neutrophils differentiated from SCF-Hoxb8 cells | D5 unstain.fcs · EV D0.fcs · EV D0.fcs-(1) · EV D5.fcs · EV D5.fcs-(1) · HA-RhoH D0.fcs · HA-RhoH D0.fcs-(1) · HA-RhoH D0.fcs-(2) · HA-RhoH D5.fcs · HA-RhoH D5.fcs-(1) · HA-RhoH D5.fcs-(2) · HA-RhoH K51A D0.fcs · HA-RhoH K51A D5.fcs · HA-RhoH unstain.fcs · K51A HA-RhoH D0.fcs · K51A HA-RhoH D0.fcs-(1) · K51A HA-RhoH D5.fcs · K51A HA-RhoH D5.fcs-(1) · K51A HA-RhoH unstain.fcs · KO D0.fcs · KO D5.fcs · RhoH KO D0.fcs · RhoH KO D0.fcs-(1) · RhoH KO D5.fcs · RhoH KO D5.fcs-(1) · RhoH KO EV D0.fcs · RhoH KO EV D5.fcs · RhoH KO EV unstain.fcs · RhoH KO unstained.fcs · WT D0 .fcs · WT D0.fcs · WT D0.fcs-(1) · WT D5.fcs · WT D5.fcs-(1) · WT D5.fcs-(2) · WT unstained.fcs · shCtrl D0.fcs · shCtrl D0.fcs-(1) · shCtrl D0.fcs-(2) · shCtrl D5.fcs · shCtrl D5.fcs-(1) · shCtrl D5.fcs-(2) · shCtrl unstain.fcs · shMyh9 D0.fcs · shMyh9 D0.fcs-(1) · shMyh9 D0.fcs-(2) · shMyh9 D5.fcs · shMyh9 D5.fcs-(1) · shMyh9 D5.fcs-(2) · shMyh9 unstain.fcs |
