Experiment Overview
| Repository ID: | FR-FCM-Z5M5 | Experiment name: | Cell Cycle analysis of HeLa cells exposed to colibactin with inhibitors | MIFlowCyt score: | 22.50% |
| Primary researcher: | Matthew Volpe | PI/manager: | Matthew Volpe | Uploaded by: | Matthew Volpe |
| Experiment dates: | 2020-10-01 - 2020-12-31 | Dataset uploaded: | Aug 2022 | Last updated: | Aug 2022 |
| Keywords: | [cell cycle] [colibactin] | Manuscripts: | |||
| Organizations: | None | ||||
| Purpose: | Cells exposed to the genotoxin colibactin, which is produced by certain strains of E. coli and other proteobacteria, typically exhibit cell cycle arrest as a result of colibactin-induced DNA damage. In this experiment, cells were exposed to either colibactin-producing E. coli, non-colibactin producing E.coli, or colibactin-producing E.coli treated with a putative colibactin-biosynthesis inhibitor. If the inhibitor is effective, those cells should not exhibit the same level of cell cycle arrest as cells which were exposed to colibactin-producing E. coli without an inhibitor. | ||||
| Conclusion: | Cells exhibited a decrease in cell-cycle analysis in a dose-dependent fashion when treated with the inhibitor, as expected. Thus we conclude that the inhibitor is interfering with colibactin biosynthesis. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
