Experiment Overview
| Repository ID: | FR-FCM-Z5MB | Experiment name: | Spectrum Flow cytometry immunophenotypic studies of human fetal intestine throughout the second trimesterby a 5-laser Cytek® Aurora | MIFlowCyt score: | 47.00% |
| Primary researcher: | Nannan Guo | PI/manager: | Nannan Guo | Uploaded by: | Nannan Guo |
| Experiment dates: | 2021-02-03 - | Dataset uploaded: | Aug 2022 | Last updated: | Aug 2022 |
| Keywords: | [human fetal intestine] [High dimensional flow cytometry] [Cytek Aurora] | Manuscripts: | |||
| Organizations: |
Leiden University Medical Center, Leiden University Medical Center, Leiden, (Netherlands)
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| Purpose: | The 26-antibody flow cytometry-based panel was developed for in-depth immunophenotyping of the major cell subsets present in the human fetal intestine through time. | ||||
| Conclusion: | Spectral flow cytometry indicated that at week 14 of gestation, the fetal intestine was mainly populated by myeloid cells and three distinct CD3–CD7+ ILCs subsets, followed by rapid appearance of adaptive CD4, CD8 T and B cell subsets in time. Ki-67+ cells were observed in all identified immune subsets as early as week 14 and up to week 22. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
