Experiment Overview

Repository ID: FR-FCM-Z5MU Experiment name: iCoreDrop development and performance MIFlowCyt score: 51.50%
Primary researcher: Holly Jensen PI/manager: Holly Jensen Uploaded by: Holly Jensen
Experiment dates: 2022-03-01 - 2022-05-31 Dataset uploaded: Aug 2022 Last updated: Sep 2022
Keywords: [autofluorescence] [spectral cytometry] [Cytek Aurora] [reference controls] [drop-in channels] [precision] Manuscripts: [36458334] Cytalogo
Organizations: Bristol Myers Squibb, Tumor Microenvironment TRC, Redwood City, CA (United States)
Purpose: Development of an immune monitoring assay with open biomarker channels using optimal spectral cytometry assay practices.
Conclusion: High assay precision is achievable on the 5-laser Cytek Aurora for a 36-color assay utilizing flexible drop in channels. A thorough interrogation of single stain controls and unmixing setup was required to achieve this result.
Comments: None
Funding: Not disclosed
Quality control: QC beads were run daily to maintain instrument performance. Similarity Indices were tracked over time for stock antibody reagents and dilutions to assess stability. Extensive bead vs cells checks were performed to determine optimal reference (single stain) controls. FMM controls were used to QC the gating strategy. Samples were run in triplicate during assay performance characterization.
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