Experiment Overview
| Repository ID: | FR-FCM-Z5MV | Experiment name: | Cross-platform immunophenotyping of human PBMC with four high-dimensional flow cytometry panels | MIFlowCyt score: | 50.88% |
| Primary researcher: | Alex Heubeck | PI/manager: | Alex Heubeck | Uploaded by: | Alex Heubeck |
| Experiment dates: | 2019-08-01 - 2020-08-01 | Dataset uploaded: | Aug 2022 | Last updated: | Nov 2022 |
| Keywords: | [Immunophenotyping] [PBMC] [spectral cytometry] [High dimensional flow cytometry] [conventional cytometry] | Manuscripts: | [36571245] |
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| Organizations: |
Allen Institute for Immunology, Seattle, Washington (United States)
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| Purpose: | To develop high parameter panels for longitudinal phenotyping and evaluate their performance on a conventional and a spectral cytometer | ||||
| Conclusion: | Utilizing multiple high parameter panels for longitudinal immunophenotyping is an effective method. Additionally, spectral and conventional cytometers provide highly similar results for the developed panels. | ||||
| Comments: | None | ||||
| Funding: | Internal | ||||
| Quality control: | Instrument QC was performed according to manufacturer recommended methods using appropriate QC beads | ||||
