Experiment Overview
| Repository ID: | FR-FCM-Z5MZ | Experiment name: | Mouse neutrophil degranulation | MIFlowCyt score: | 53.75% |
| Primary researcher: | Shuang Peng | PI/manager: | Shuang Peng | Uploaded by: | Shuang Peng |
| Experiment dates: | 2017-10-02 - 2022-05-30 | Dataset uploaded: | Aug 2022 | Last updated: | Aug 2022 |
| Keywords: | [CD63] [degranulation] [Mouse neutrophil] | Manuscripts: | |||
| Organizations: |
The University of Bern, Bern, (Switzerland)
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| Purpose: | To examine mouse neutrophil degranulation by measuring cell surface expression of CD63. | ||||
| Conclusion: | RhoH incuction by GM-CSF or RhoH overexpression impaired neutrophil degranulation. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | PE-conjugated Rat IgG2a, κ isotype control (clone R35-95; BD Biosciences) was used at the same concentration. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · BM neutrophils | IgG GM 20 min+C5a.fcs · IgG GM 3h+C5a.fcs · Isotype GM20 +c5a.fcs · Isotype GM3h +C5a.fcs · Isotype control GM 20 min+C5a.fcs · Isotype control GM 3h+C5a.fcs · KO 10.fcs · KO 20.fcs · KO Control.fcs · KO Control.fcs-(1) · KO GM + c5a.fcs · KO GM 180 min +C5a.fcs · KO GM 20 min +C5a.fcs · KO GM 20min+C5a.fcs · KO GM 3h +C5a.fcs · KO GM 3h +c5a.fcs · KO GM 3h.fcs · KO GM+C5a.fcs · KO GM+C5a.fcs-(1) · KO PMA.fcs · KO PMA.fcs-(1) · KO control.fcs · KO control.fcs-(1) · KO medium.fcs · KO unstimulated.fcs · Mice KO Control.fcs · Mice KO GM+C5a.fcs · Mice KO PMA.fcs · Mice WT GM+C5a.fcs · Mice WT PMA.fcs · Mice WT control.fcs · RhoH-_- GM+C5a.fcs · RhoH-_- PMA.fcs · RhoH-_- control .fcs · Tube_001.fcs · Tube_002.fcs · Tube_003.fcs · Tube_004.fcs · Tube_005.fcs · Tube_006.fcs · Vector control .fcs · WT 10.fcs · WT 20.fcs · WT Control.fcs · WT Control.fcs-(1) · WT GM + C5a.fcs · WT GM 180 min +C5a.fcs · WT GM 20 min +C5a.fcs · WT GM 3h + C5a.fcs · WT GM 3h+C5a.fcs · WT GM 3h.fcs · WT GM+C5a.fcs · WT GM+C5a.fcs-(1) · WT PMA.fcs · WT PMA.fcs-(1) · WT control.fcs · WT control.fcs-(1) · WT medium.fcs · WT unstimulated.fcs · unstained.fcs |
| · Hoxb8 neutrophils | GM+C5a IgG Hoxb8.fcs · GM+C5a.fcs · HA-RhoH control .fcs · HA-RhoH Control .fcs · HA-RhoH GM+C5a.fcs · HA-RhoH GM+C5a.fcs-(1) · HA-RhoH PMA.fcs · HA-RhoH PMA.fcs-(1) · HA-RhoH unstimulated.fcs · IgG Hoxb8 GM+C5a.fcs · IgG Hoxb8 PMA.fcs · IgG Hoxb8 unstimulated.fcs · IgG control GM+C5a.fcs · IgG control PMA.fcs · IgG control unstimulated.fcs · IgG unstimulated.fcs · Isotype contol unstimulated.fcs · Isotype control.fcs · KO+RhoH GM+C5a.fcs · KO+RhoH PMA.fcs · KO+RhoH unstimulated.fcs · PMA IgG Hoxb8 .fcs · PMA.fcs · Vector GM+C5a.fcs · Vector PMA.fcs · unstimulated IgG Hoxb8.fcs · unstimulated.fcs |
| Individuals | |
|---|---|
| · isotype control | GM+C5a IgG Hoxb8.fcs · IgG GM 20 min+C5a.fcs · IgG GM 3h+C5a.fcs · IgG Hoxb8 GM+C5a.fcs · IgG Hoxb8 PMA.fcs · IgG Hoxb8 unstimulated.fcs · IgG control GM+C5a.fcs · IgG control PMA.fcs · IgG control unstimulated.fcs · IgG unstimulated.fcs · Isotype GM20 +c5a.fcs · Isotype GM3h +C5a.fcs · Isotype contol unstimulated.fcs · Isotype control GM 20 min+C5a.fcs · Isotype control GM 3h+C5a.fcs · Isotype control.fcs · PMA IgG Hoxb8 .fcs · unstained.fcs · unstimulated IgG Hoxb8.fcs |
| · control | HA-RhoH control .fcs · HA-RhoH Control .fcs · HA-RhoH unstimulated.fcs · KO Control.fcs · KO Control.fcs-(1) · KO control.fcs · KO control.fcs-(1) · KO medium.fcs · KO unstimulated.fcs · KO+RhoH unstimulated.fcs · Mice KO Control.fcs · Mice WT control.fcs · RhoH-_- control .fcs · Tube_001.fcs · Tube_004.fcs · Vector control .fcs · WT Control.fcs · WT Control.fcs-(1) · WT control.fcs · WT control.fcs-(1) · WT medium.fcs · WT unstimulated.fcs · unstimulated.fcs |
| · activated | GM+C5a.fcs · HA-RhoH GM+C5a.fcs · HA-RhoH GM+C5a.fcs-(1) · HA-RhoH PMA.fcs · HA-RhoH PMA.fcs-(1) · KO 10.fcs · KO 20.fcs · KO GM + c5a.fcs · KO GM 180 min +C5a.fcs · KO GM 20 min +C5a.fcs · KO GM 20min+C5a.fcs · KO GM 3h +C5a.fcs · KO GM 3h +c5a.fcs · KO GM 3h.fcs · KO GM+C5a.fcs · KO GM+C5a.fcs-(1) · KO PMA.fcs · KO PMA.fcs-(1) · KO+RhoH GM+C5a.fcs · KO+RhoH PMA.fcs · Mice KO GM+C5a.fcs · Mice KO PMA.fcs · Mice WT GM+C5a.fcs · Mice WT PMA.fcs · PMA.fcs · RhoH-_- GM+C5a.fcs · RhoH-_- PMA.fcs · Tube_002.fcs · Tube_003.fcs · Tube_005.fcs · Tube_006.fcs · Vector GM+C5a.fcs · Vector PMA.fcs · WT 10.fcs · WT 20.fcs · WT GM + C5a.fcs · WT GM 180 min +C5a.fcs · WT GM 20 min +C5a.fcs · WT GM 3h + C5a.fcs · WT GM 3h+C5a.fcs · WT GM 3h.fcs · WT GM+C5a.fcs · WT GM+C5a.fcs-(1) · WT PMA.fcs · WT PMA.fcs-(1) |
