Experiment Overview
| Repository ID: | FR-FCM-Z5NY | Experiment name: | Vakrakou A., Paschalidis N., et al 2023 Deep immunophenotyping of PBMCs from MS patients | MIFlowCyt score: | 83.75% |
| Primary researcher: | Nikolaos Paschalidis | PI/manager: | Nikolaos Paschalidis | Uploaded by: | Nikolaos Paschalidis |
| Experiment dates: | 2021-10-01 - 2022-01-31 | Dataset uploaded: | Aug 2022 | Last updated: | Jan 2023 |
| Keywords: | [B cells] [NK cells] [T cells] [mass cytometry] [macrophages] [multiple sclerosis] [tumefactive multiple sclerosis] [myeloid-signature] | Manuscripts: | |||
| Organizations: |
Biomedical Research Foundation Academy of Athens, Mass Cytometry - CyTOF Laboratory, Athens, (Greece)
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| Purpose: | We designed a novel 44 -parameter mass cytometry panel to interrogate the role of effector and regulatory subpopulations of peripheral blood myeloid subsets along with B and T cells (excluding granulocytes) in MS, assessing three different patient cohorts: RRMS, PPMS (Primary Progressive) and Tumefactive MS patients (TMS) | ||||
| Conclusion: | We have identified previously uncharacterized subsets of circulating myeloid cells and shown them to correlate with distinct disease forms of MS as well as with specific disease state (relapse / remission). These results highlight the potential for therapies that specifically target myeloid cells to impact MS activity and progression depending on the disease subtype and stage of the disease. | ||||
| Comments: | None | ||||
| Funding: | This study was funded by the Special Account for Research Funding (E.L.K.E., grant number 10563). This work was further supported by the “Infrastructure for Preclinical and early-Phase Clinical Development of Drugs, Therapeutics and Biomedical Devices (EATRIS-GR)” (MIS 5028091), which is implemented under the Action “Reinforcement of the Research and Innovation Infrastructure”, funded by the Operational Programme “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014- 2020) and co-financed by Greece and the European Union (European Regional Development Fund). EAN fellowship was granted to AGV. The funders were not involved in the study design, collection, analysis, interpretation of data, the writing of this article or the decision to submit it for publication. | ||||
| Quality control: | An optimized multiplex flow cytometry protocol for the analysis of intracellular signaling in peripheral blood mononuclear cells | Data acquisition quality was monitored through QC - tuning testing of the Helios mass cytometer | |||
Experiment variables
| Conditions | |
|---|---|
| · HD | AV01.fcs · AV02.fcs · AV03.fcs · AV04.fcs · AV19.fcs · AV20.fcs · AV33.fcs · AV34.fcs · AV35.fcs · AV36.fcs |
| · PPMS | AV05.fcs · AV06.fcs · AV08.fcs · AV21.fcs · AV22.fcs · AV23.fcs · AV24.fcs · AV42.fcs · AV43.fcs · AV44.fcs |
| · RRMS | AV14.fcs · AV15.fcs · AV16.fcs · AV30.fcs · AV31.fcs · AV32.fcs · AV45.fcs · AV46.fcs · AV47.fcs · AV48.fcs |
| · TMS | AV07.fcs · AV09.fcs · AV10.fcs · AV11.fcs · AV12.fcs · AV13.fcs · AV25.fcs · AV26.fcs · AV28.fcs · AV29.fcs · AV37.fcs · AV38.fcs · AV39.fcs · AV40.fcs · AV41.fcs |
