
Experiment Overview
Repository ID: | FR-FCM-Z5P8 | Experiment name: | Group-specific phytoplankton carbon fixation rate in the southwest Pacific Ocean | MIFlowCyt score: | 21.97% |
Primary researcher: | Denise Rui Ying Ong | PI/manager: | Adriana Lopes dos Santos | Uploaded by: | Denise Rui Ying Ong |
Experiment dates: | 2022-09-11 - | Dataset uploaded: | Sep 2022 | Last updated: | Oct 2022 |
Keywords: | [marine] [phytoplankton] [Chatham rise] [carbon isotope incubation] [southwest Pacific ocean] | Manuscripts: | |||
Organizations: |
Nanyang Technological University, Singapore, (Singapore)
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Purpose: | Flow cytometry sorted populations of 1) marine Synechococcus, 2) photosynthetic picoeukaryotes and 3) photosynthetic nanoeukaryotes. Seawater samples were collected during TAN1810 oceanography campaign (21st October - 21st November 2018) at Southwest Pacific Ocean, near the Chatham Rise (New Zealand). There are three different sample types collected: 1) Initial- Water samples were fixed and sorted at time of collection 2) Light - water samples were spiked with radioactively labelled bicarbonate and incubated in-situ. Three replicates per station labelled vials A, B, C. 3) Dark - water samples were spiked with radioactively labelled bicarbonate and incubated in the dark. | ||||
Conclusion: | None | ||||
Comments: | Flow cytometry file metadata is available on Github at (https://github.com/deniseong/marine-Synechococcus-metaB/tree/main/7_FCM%20files%20metadata). Samples were collected by Andres Gutiérrez-Rodríguez (Instituto Español de Oceanografía Centro Oceanográfico de Xixón) and Adriana Lopes dos Santos (Nanyang Technological University, Asian School of the Environment). Flow cytometry sorting was carried out by Dominique Marie (Sorbonne Université, CNRS, UMR 7144 (AD2M), Station Biologique de Roscoff) and Adriana Lopes dos Santos. | ||||
Funding: | Not disclosed | ||||
Quality control: | None |