Experiment Overview
| Repository ID: | FR-FCM-Z5Q8 | Experiment name: | 32 marker mass cytometry panel for T cells | MIFlowCyt score: | 46.58% |
| Primary researcher: | Alexis Sponaugle | PI/manager: | Alexis Sponaugle | Uploaded by: | Alexis Sponaugle |
| Experiment dates: | 2021-08-10 - 2022-10-10 | Dataset uploaded: | Sep 2022 | Last updated: | Oct 2022 |
| Keywords: | [CD4 T cells] [mass cytometry] [CD8 T cells] | Manuscripts: | |||
| Organizations: |
University of North Carolina at Chapel Hill, Chapel Hill, (United States of America)
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| Purpose: | To standardize a 32 marker mass cytometry panel able to characterize CD4 and CD8 T cells. | ||||
| Conclusion: | This panel is able to identify all canonical lineage markers as well as CD4 T cell helper subsets, CD4 and CD8 T cell activation and memory subsets | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | The same human PBMCs were spiked into all fully stained samples tagged with CD45 111Cd in order to confirm optimal staining and account for batch effects. | ||||
