Experiment Overview
| Repository ID: | FR-FCM-Z5RA | Experiment name: | EP20201103_CyTOF analysis_anti-THBS1 treatment of MuSC in culture | MIFlowCyt score: | 65.75% |
| Primary researcher: | Ermelinda Porpiglia | PI/manager: | Helen Blau | Uploaded by: | Ermelinda Porpiglia |
| Experiment dates: | 2020-11-03 - 2020-11-03 | Dataset uploaded: | Oct 2022 | Last updated: | Nov 2022 |
| Keywords: | [IdU] [Young and Aged] [Thrombospondin-1 blockade] [Molecular Profile] | Manuscripts: | |||
| Organizations: |
Stanford University, Baxter Laboratory for Stem Cell Biology, Stanford, CA (United States)
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| Purpose: | To determine the molecular response of sorted young and aged muscle stem cells to thrombospondin-1 blockade in vitro. | ||||
| Conclusion: | Thrombospondin-1 blockade in vitro leads to an increase in the proliferation of wild type young and aged muscle stem cells but not CD47-/- muscle stem cells | ||||
| Comments: | CyTOF molecular profile of young and aged muscle stem cells in response to in vitro thrombospondin-1 blockade | ||||
| Funding: | This study was supported by BD Biosciences Stem Cell grant (E.P.); NIH grants NS089533 and AG020961, CIRM grant RB5-07469 and the Baxter Foundation (H.M.B.). | ||||
| Quality control: | Visual inspection of biaxial plots | ||||
Experiment variables
| Conditions | |
|---|---|
| · control | CD47 KO Old Ctr-1.fcs · CD47 KO Old Ctr-2.fcs · CD47 KO Old Ctr-3.fcs · Old Ctr-1.fcs · Old Ctr-2.fcs · Old Ctr-3.fcs · Yng Ctr-1.fcs · Yng Ctr-2.fcs · Yng Ctr-3.fcs |
| · anti THBS1 | CD47 KO Old anti-THBS1-1.fcs · CD47 KO Old anti-THBS1-2.fcs · CD47 KO Old anti-THBS1-3.fcs · Old anti-THBS1-1.fcs · Old anti-THBS1-2.fcs · Old anti-THBS1-3.fcs · Yng anti-THBS1-1.fcs · Yng anti-THBS1-2.fcs · Yng anti-THBS1-3.fcs |
| Sample Type | |
|---|---|
| · WT Young | Yng Ctr-1.fcs · Yng Ctr-2.fcs · Yng Ctr-3.fcs · Yng anti-THBS1-1.fcs · Yng anti-THBS1-2.fcs · Yng anti-THBS1-3.fcs |
| · WT Aged | Old Ctr-1.fcs · Old Ctr-2.fcs · Old Ctr-3.fcs · Old anti-THBS1-1.fcs · Old anti-THBS1-2.fcs · Old anti-THBS1-3.fcs |
| · CD47KO Aged | CD47 KO Old Ctr-1.fcs · CD47 KO Old Ctr-2.fcs · CD47 KO Old Ctr-3.fcs · CD47 KO Old anti-THBS1-1.fcs · CD47 KO Old anti-THBS1-2.fcs · CD47 KO Old anti-THBS1-3.fcs |
