Experiment Overview
| Repository ID: | FR-FCM-Z5X6 | Experiment name: | Maternal diet and gut microbiome composition modulate early life immune development | MIFlowCyt score: | 62.02% |
| Primary researcher: | Erica Grant | PI/manager: | Erica Grant | Uploaded by: | Erica Grant |
| Experiment dates: | 2019-09-01 - 2022-09-01 | Dataset uploaded: | Aug 2022 | Last updated: | Apr 2023 |
| Keywords: | [neonate ] [Gut microbiome] [Early-T cells] [Dietary fiber] | Manuscripts: | |||
| Organizations: |
Luxembourg Institute of Health, Quantitative Biology Unit, Esch-sur-Alzette, (Luxembourg)
Luxembourg Institute of Health, Department of Infection and Immunity, Esch-sur-Alzette, (Luxembourg) |
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| Purpose: | Early life is a critical period for the development of the newborn intestine, which undergoes microbiome colonization concomitant with the maturation of the mucus barrier and the immune system. As dietary fiber exerts a powerful impact on both the microbiome and immune function in adult mice, we sought to understand its role in early life by breeding mice colonized with a 13 or 14-member synthetic microbiome community (13SM or 14SM), fed a fiber-rich (FR) or fiber-free (FF) diet. We then analysed the proportion of innate and adaptive immune cells at 15 days of age, during weaning. | ||||
| Conclusion: | Maternal fiber deprivation and microbiome significantly impacted the proportions of innate and adaptive immune cell subsets, especially skewing on the type 2 versus type 3 proportions in the presence or absence of Akkermansia (i.e. 13SM or 14SM). | ||||
| Comments: | NA | ||||
| Funding: | Luxembourg National Research Fund (FNR) for funding this research through CORE grants (C15/BM/10318186 and C18/BM/12585940); FNR PRIDE (17/11823097), Fondation du Pélican de Mie et Pierre Hippert-Faber, under the aegis of the Fondation de Luxembourg; European Commission Horizon 2020 Marie Skłodowska-Curie Actions individual fellowship (897408); Luxembourg's Ministry of Higher Education and Research (MESR) funding supporting the National Cytometry Platform (NCP). | ||||
| Quality control: | FMO, compensation samples | ||||
Experiment variables
| Conditions | |
|---|---|
| · 13SM FR | Tcells_samples_A7_14SMb-9.fcs · Tcells_samples_B7_14SMb-10.fcs · Tcells_samples_D7_13SM-FR-1.2.fcs · Tcells_samples_D7_14SMb-12.fcs · Tcells_samples_E7_13SM-FR-1.3.fcs · Tcells_samples_F7_13SM-FR-1.4.fcs · Tcells_samples_F7_14SMb-14.fcs · Tcells_samples_G7_13SM-FR-1.5.fcs · Tcells_samples_G7_14SMb-15.fcs · Tcells_samples_H7_14SMb-16.fcs · Tcells_samples_E7_14SMb-13.fcs · Tcells_samples_C7_13SM-FR-1.1.fcs |
| · 14SM FF | Tcells_samples_A4_Colon-1-FF.fcs · Tcells_samples_B4_Colon-2-FF.fcs · Tcells_samples_C4_Colon-3-FF.fcs · Tcells_samples_D4_Colon-4-FF.fcs · Tcells_samples_H3_14SM-FF-8.fcs · Tcells_samples_C4_14SM-FF-11.fcs · Tcells_samples_B4_14SM-FF-10.fcs · Tcells_samples_A4_14SM-FF-9.fcs |
| · 14SM FR | Tcells_samples_A6_14SM-1.fcs · Tcells_samples_B6_14SM-2.fcs · Tcells_samples_C6_14SM-3.fcs · Tcells_samples_D6_14SM-4.fcs · Tcells_samples_E6_14SM-5.fcs · Tcells_samples_F6_14SM-6.fcs · Tcells_samples_G6_14SM-7.fcs · Tcells_samples_H6_14SM-8.fcs · Tcells_samples_A3_14SM-FR-1.fcs · Tcells_samples_B3_14SM-FR-2.fcs · Tcells_samples_C3_14SM-FR-3.fcs · Tcells_samples_D3_14SM-FR-4.fcs · Tcells_samples_E3_14SM-FR-5.fcs · Tcells_samples_F3_14SM-FR-6.fcs · Tcells_samples_G3_14SM-FR-7.fcs · Tcells_samples_C7_14SMb-11.fcs |
| · GF FF | MUNING_A7_Colon-1.fcs · MUNING_A8_Colon-8.fcs · MUNING_B7_Colon-2.fcs · MUNING_B8_Colon-9.fcs · MUNING_C7_Colon-3.fcs · MUNING_C8_Colon-10.fcs · MUNING_D7_Colon-4.fcs · MUNING_D8_Colon-11.fcs · MUNING_E7_Colon-5.fcs · MUNING_E8_Colon-12.fcs · MUNING_F7_Colon-6.fcs · MUNING_F8_Colon-13.fcs · MUNING_G7_Colon-7.fcs · T cells_A3_Colon 1 GF FF.fcs · T cells_B3_Colon 2 GF FF.fcs · T cells_C3_Colon 3 GF FF.fcs · T cells_D3_Colon 4 GF FF.fcs · T cells_E3_Colon 5 GF FF.fcs · T cells_F3_Colon 6 GF FF.fcs · T cells_G3_Colon 7 GF FF.fcs |
| · GF FR | T cells_A4_Colon 9 GF FR.fcs · T cells_C4_Colon 11 GF FR.fcs · T cells_D4_Colon 12 GF FR.fcs · T cells_H3_Colon 8 GF FR.fcs · Tcells_samples_C4_Sample6.fcs · Tcells_samples_D3_Sample7.fcs · Tcells_samples_D4_Sample8.fcs · Tcells_samples_E3_Sample9.fcs · Tcells_samples_F3_Sample10.fcs · Tcells_samples_G3_Sample11.fcs · Tcells_samples_H3_Sample12.fcs · T cells_B4_Colon 10 GF FR.fcs · Tcells_samples_B3_Sample3.fcs · Tcells_samples_B4_Sample4.fcs · Tcells_samples_A3_Sample1.fcs · Tcells_samples_C3_Sample5.fcs · Tcells_samples_A4_Sample2.fcs |
| · FMO | Tcells_FMOs_A4_NKp46.fcs · Tcells_FMOs_B4_Foxp3.fcs · Tcells_FMOs_C4_Gata3.fcs · Tcells_FMOs_D4_Tbet.fcs · Tcells_FMOs_E4_RORg.fcs |
| Sample Type | |
|---|---|
| · FMO | Tcells_FMOs_A4_NKp46.fcs · Tcells_FMOs_B4_Foxp3.fcs · Tcells_FMOs_C4_Gata3.fcs · Tcells_FMOs_D4_Tbet.fcs · Tcells_FMOs_E4_RORg.fcs |
| · Colonic lamina propria | MUNING_A7_Colon-1.fcs · MUNING_A8_Colon-8.fcs · MUNING_B7_Colon-2.fcs · MUNING_B8_Colon-9.fcs · MUNING_C7_Colon-3.fcs · MUNING_C8_Colon-10.fcs · MUNING_D7_Colon-4.fcs · MUNING_D8_Colon-11.fcs · MUNING_E7_Colon-5.fcs · MUNING_E8_Colon-12.fcs · MUNING_F7_Colon-6.fcs · MUNING_F8_Colon-13.fcs · MUNING_G7_Colon-7.fcs · T cells_A3_Colon 1 GF FF.fcs · T cells_A4_Colon 9 GF FR.fcs · T cells_B3_Colon 2 GF FF.fcs · T cells_C3_Colon 3 GF FF.fcs · T cells_C4_Colon 11 GF FR.fcs · T cells_D3_Colon 4 GF FF.fcs · T cells_D4_Colon 12 GF FR.fcs · T cells_E3_Colon 5 GF FF.fcs · T cells_F3_Colon 6 GF FF.fcs · T cells_G3_Colon 7 GF FF.fcs · T cells_H3_Colon 8 GF FR.fcs · Tcells_samples_A4_Colon-1-FF.fcs · Tcells_samples_A6_14SM-1.fcs · Tcells_samples_A7_14SMb-9.fcs · Tcells_samples_B4_Colon-2-FF.fcs · Tcells_samples_B6_14SM-2.fcs · Tcells_samples_B7_14SMb-10.fcs · Tcells_samples_C4_Colon-3-FF.fcs · Tcells_samples_C4_Sample6.fcs · Tcells_samples_C6_14SM-3.fcs · Tcells_samples_D3_Sample7.fcs · Tcells_samples_D4_Colon-4-FF.fcs · Tcells_samples_D4_Sample8.fcs · Tcells_samples_D6_14SM-4.fcs · Tcells_samples_D7_13SM-FR-1.2.fcs · Tcells_samples_D7_14SMb-12.fcs · Tcells_samples_E3_Sample9.fcs · Tcells_samples_E6_14SM-5.fcs · Tcells_samples_E7_13SM-FR-1.3.fcs · Tcells_samples_F3_Sample10.fcs · Tcells_samples_F6_14SM-6.fcs · Tcells_samples_F7_13SM-FR-1.4.fcs · Tcells_samples_F7_14SMb-14.fcs · Tcells_samples_G3_Sample11.fcs · Tcells_samples_G6_14SM-7.fcs · Tcells_samples_G7_13SM-FR-1.5.fcs · Tcells_samples_G7_14SMb-15.fcs · Tcells_samples_H3_Sample12.fcs · Tcells_samples_H6_14SM-8.fcs · Tcells_samples_H7_14SMb-16.fcs · Tcells_samples_E7_14SMb-13.fcs · T cells_B4_Colon 10 GF FR.fcs · Tcells_samples_C7_13SM-FR-1.1.fcs · Tcells_samples_A3_14SM-FR-1.fcs · Tcells_samples_B3_14SM-FR-2.fcs · Tcells_samples_B3_Sample3.fcs · Tcells_samples_B4_Sample4.fcs · Tcells_samples_C3_14SM-FR-3.fcs · Tcells_samples_D3_14SM-FR-4.fcs · Tcells_samples_E3_14SM-FR-5.fcs · Tcells_samples_F3_14SM-FR-6.fcs · Tcells_samples_G3_14SM-FR-7.fcs · Tcells_samples_H3_14SM-FF-8.fcs · Tcells_samples_C4_14SM-FF-11.fcs · Tcells_samples_A3_Sample1.fcs · Tcells_samples_B4_14SM-FF-10.fcs · Tcells_samples_C3_Sample5.fcs · Tcells_samples_A4_14SM-FF-9.fcs · Tcells_samples_A4_Sample2.fcs · Tcells_samples_C7_14SMb-11.fcs |
